Review

32 KKU Science Journal Volume 39 Number 1 Review
µ“√“ß∑’Ë 1 · ¥ß°“√‡ª√’¬∫‡∑’¬∫‡∑§π‘§°“√°”®—¥‚ª√µ’π∑’Ë¡’ª√‘¡“≥¡“°
‡∑§π‘§
Cibacron blue gels
Immunoaffinity depletion Equalizer bead
¢âÕ‡ª√’¬∫‡∑’¬∫
method
technology
À≈—°°“√ affinity antibody affinity affinity
§«“¡®”‡æ“– µË” Ÿß ª“π°≈“ß
√–¬–‡«≈“ 1-2 —Ë«‚¡ß ¡“°°«à“ 5 —Ë«‚¡ß 1-2 —Ë«‚¡ß
ª√‘¡“≥µ—«Õ¬à“ß πâÕ¬ πâÕ¬ πâÕ¬
π‘¥µ—«Õ¬à“ß ´’√—¡ ´’√—¡ ´’√—¡
‘Èπ‡π◊ÈÕ
“√‡À≈«„π√à“ß°“¬Õ◊ËπÊ
√“§“ ∂Ÿ° ·æß ª“π°≈“ß
°“√·¬àß®—∫¢Õß‚ª√µ’π∑’˵âÕß°“√ (enrichment
techniques)
The equalizer bead technology
°“√ —߇§√“–Àå “¬‚æ≈’‡ªª‰∑¥å∑’Ë “¡“√∂
®—∫°—∫‚ª√µ’π‰¥âÕ¬à“ßÀ≈“°À≈“¬ ´÷Ë߇°‘¥®“°°“√
ÕÕ°·∫∫ “¬‚æ≈’‡ªª‰∑¥åπ‘¥ ç‡Œ°´–‡ªª‰∑¥åé ∑’Ë
®– “¡“√∂ √â“ßµâπ·∫∫¢Õß‚ª√µ’π‰¥â¡“°∂÷ß
64,000,000 π‘¥ (Boschetti et al., 2008) ”À√—∫
“¬‚æ≈’‡ªª‰∑¥å¥—ß°≈à“«®–∂Ÿ°π”¡“µ√÷߉«â∫π
‡¡Á¥æ≈“ µ‘° (sepharose) ¥â«¬æ—π∏–‚§«“‡≈πµå ‡√’¬°
equalizer beads „πÕ—µ√“ à«π∑’ˇ∑à“°—π®– “¡“√∂®—∫°—∫
‚ª√µ’π∑ÿ°π‘¥‰¥â„πª√‘¡“≥‡∑à“°—π (Boschetti et al.,
2008) ‡√’¬° equivalent binding capacity ´÷Ë߇¡◊ËÕπ”
‚ª√µ’π∑’Ë®—∫Õ¬Ÿà∫π‡¡Á¥æ≈“ µ‘°ÕÕ°¡“∑”°“√«‘‡§√“–Àå
°Á®–æ∫«à“ “¡“√∂≈¥°“√∫¥∫—ߢÕß‚ª√µ’π∑’Ë¡’ª√‘¡“≥
¡“°·≈–¬—ß∑”„À₪√µ’π∑’Ë¡’ª√‘¡“≥πâÕ¬∂Ÿ°µ√«®æ∫
¥â«¬°≈‰°¥—ß°≈à“«®–∑”„Àâ “√∫àß’È∑“ß’«¿“æπ‘¥
„À¡àÊ ∂Ÿ°§âπæ∫‰¥âßà“¬¡“°¢÷Èπ (√Ÿª∑’Ë 4) ®“°°“√
»÷°…“„π‡∫◊ÈÕßµâπ »‘√‘æ√ ¿—∑√°‘®°”®√ ·≈–§≥–
(unpublished data) æ∫«à“·∫∫·ºπ 2DE ¢Õß‚ª√µ’π„π
´’√—¡∑’ˉ¥â®“°°“√‡µ√’¬¡¥â«¬«‘∏’π’È ¡’§«“¡·µ°µà“ß®“°
Õ’° 2 «‘∏’¢â“ßµâπ
”À√—∫‡∑§π‘§π’È “¡“√∂ª√–¬ÿ°µå„â°—∫
µ—«Õ¬à“ßπ‘¥Õ◊ËπÊ ∑’Ë¡’§«“¡ ”§—≠∑“ß°“√·æ∑¬å ‡àπ
“√‡À≈«„π√à“ß°“¬, ‘Èπ‡π◊ÈÕ ·≈–‡´≈≈å‡æ“–‡≈’È¬ß ‡ªìπµâπ
√«¡∑—Èß “¡“√∂𔉪ª√–¬ÿ°µå„â„π°“√·¬°‚ª√µ’ππ‘¥
recombinant DNA product Õ’°¥â«¬ (Boschetti et al.,
2008) ∑—Èßπ’ȉ¡à·π–π”°“√„âæ≈“ ¡“„π°“√»÷°…“
‡π◊ËÕß®“°Õ“®‡°‘¥°“√‡°“–°≈ÿà¡¢Õß‚ª√µ’π πÕ°®“°π’Ȭ—ß
¡’¢âÕ®”°—¥§◊Õ °“√®—∫°—π¢Õß‚ª√µ’π·≈– equalizer
beads ®–®—∫¥â«¬æ—π∏–ÕàÕπÊ Õ“®∑”„ÀâÀ≈ÿ¥ÕÕ°®“°
°—π‰¥âßà“¬ (Thulasiraman et al., 2005; Boschetti
et al., 2008)