Review

«.«‘∑¬. ¡¢. 39(1) 113-119 (2554) KKU Sci. J.39(1) 113-119 (2011)
Effect of Chitosan on Growth and Development of
Phalaenopsis cornucervi (Breda) Blume & Rchb.f.
Somporn Prasertsongskun 1* Witool Chaipakdee 1
∫∑§—¥¬àÕ
‡µ√’¬¡‰§‚µ´“π®“°·°πÀ¡÷° ‚¥¬¥—¥·ª≈ß®“°«‘∏’¢Õß Alimunair ·≈– Zainuddin (1992) ‰¥âª√‘¡“≥
‰§‚µ´“π√âÕ¬≈– 20 (w/w) ‡¡◊ËÕπ”‚æ√‚∑§Õ√å¡¢Õß°≈⫬‰¡â‡Õ◊ÈÕ߇¢“°«“ßÕàÕπ ¡“‡æ“–‡≈’Ȭ߄πÕ“À“√‡À≈« Ÿµ√
VW (Vacin and Went, 1949) ∑’ˇµ‘¡‰§‚µ´“π∑’ˉ¥â®“°·°πÀ¡÷° §«“¡‡¢â¡¢âπ 5 10 15 20 ·≈– 25 ¡‘≈≈‘°√—¡/
≈‘µ√ ‡ªìπ‡«≈“ 4 —ª¥“Àå æ∫«à“‰§‚µ´“π∑ÿ°√–¥—∫§«“¡‡¢â¡¢âπ°√–µÿâπ°“√‡æ‘Ë¡ª√‘¡“≥¢Õß‚æ√‚∑§Õ√å¡ ‡¡◊ËÕ‡ª√’¬∫
‡∑’¬∫°—∫ÿ¥§«∫§ÿ¡∑’ˉ¡à‡µ‘¡‰§‚µ´“π ‡¡◊ËÕπ”‚æ√‚∑§Õ√å¡¡“‡æ“–‡≈’Ȭ߄πÕ“À“√·¢Áß æ∫«à“‰§‚µ´“π°√–µÿâπ°“√
‡®√‘≠‡µ‘∫‚µ¢Õß‚æ√‚∑§Õ√塉¥âÕ¬à“ß¡’π—¬ ”§—≠ ‚¥¬‡©æ“–‰§‚µ´“𧫓¡‡¢â¡¢âπ 15 ¡‘≈≈‘°√—¡/≈‘µ√ „Àâ®”π«π
„∫ (4.3 „∫/µâπ) ®”π«π√“° (2.5 √“°/µâπ) ·≈–®”π«πÀπàÕ (1.5 ÀπàÕ/µâπ) ‡©≈’ˬ Ÿß∑’Ë ÿ¥ Õ¬à“߉√°Áµ“¡‡¡◊ËÕ„Àâ
‰§‚µ´“𧫓¡‡¢â¡¢âπ Ÿß¢÷Èπ ®–¬—∫¬—Èß°“√‡®√‘≠‡µ‘∫‚µ¢Õß‚æ√‚∑§Õ√å¡ µâπ°≈â“∑’ˇ®√‘≠‡µ‘∫‚µ‡¡◊ËÕπ”¬â“¬ÕÕ°ª≈Ÿ°„π
«— ¥ÿª≈Ÿ°∑’ˇªìπ∂à“π·≈–√¥¥â«¬‰§‚µ´“𧫓¡‡¢â¡¢âπµà“ßÊ ‡ªìπ‡«≈“ 4 —ª¥“Àå æ∫«à“µâπ°≈â“¡’’«‘µ√Õ¥¡“°°«à“
√âÕ¬≈– 50
Abstract
The chitosan was prepared from cuttlebone by the modified method of Alimunair and Zainuddin
(1992) with the yield of 20% (w/w). Protocorms of Phalaenopsis cornucervi (Breda) Blume & Rchb.f. were
cultured in VW (Vacin and Went, 1949) liquid medium added with chitosan from cuttlebone at five various
concentrations of 5, 10, 15, 20 and 25 mg/l for 4 weeks. All chitosan concentrations could enhance
protocorm production when compared to the control without chitosan. Additionally, protocorms were also
cultured on VW semi-solid medium. The results showed the highest number of leaves (4.3 leaves), roots (2.5
roots) and shoots (1.5 shoots) could obtain when 15 mg/l of chitosan was added to the VW semi-solid
medium. However, chitosan at higher concentration showed the inhibitory effect on the growth of protocorm.
After the regenerated plantlets were potted in charcoal and watered with various concentrations of chitosan
for 4 weeks, more than 50% were of them survived.
Keywords : Chitosan, Phalaenopsis, Protocorm
1
Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand 94000
* Corresponding Author, E-mail : psomporn@bunga.pn.psu.ac.th