Review

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22 KKU Science Journal Volume 39 Number 1 Review
√“¬ß“𧫓¡ ”‡√Á®¢Õß°“√ àß∂à“¬¬’π„πæ◊
„∫‡≈’Ȭ߇¥’ˬ«
°“√∂à “¬∑Õ¥¬’ π‚¥¬„â ·∫§∑’ ‡√’ ¬
Agrobacterium ‰¥âº≈¥’ ·≈–¡’ª√– ‘∑∏‘¿“æ„π°“√ àß
∂à“¬¬’π Ÿß„πæ◊„∫‡≈’ȬߧŸà ‰¡à§àÕ¬‰¥âº≈°—∫æ«°∏—≠æ◊
√«¡∂÷ßæ«°æ◊„∫‡≈’Ȭ߇¥’ˬ«π‘¥Õ◊Ëπ ·µàÕ¬à“߉√°Áµ“¡
°Á‰¥â¡’°“√∑¥≈Õß„â·∫§∑’‡√’¬ Agrobacterium „π°“√
àß∂à“¬¬’π‡¢â“ Ÿàæ◊„∫‡≈’Ȭ߇¥’ˬ«‰¥â‡ªìπº≈ ”‡√Á®·≈â«„πæ◊
„∫‡≈’Ȭ߇¥’ˬ«∫“ßπ‘¥ (Conner and Dommisse, 1992;
Rashid et al., 1996) ‚¥¬‡©æ“– Rashid et al. (1996)
√“¬ß“π°“√ àß∂à“¬¬’π‡¢â“ Ÿà¢â“« indica æ∫«à“ “¡“√∂
àß∂à“¬‰¥â‡ªìπº≈ ”‡√Á®·≈– “¡“√∂∂à“¬∑Õ¥‰ª àß√ÿàπ T1
‰¥â ·≈–¡’°“√°√–®“¬¢Õ߬’π‡ªìπ‰ªµ“¡°Æ¢Õ߇¡π‡¥≈
µàÕ¡“„πªï Wang et al. (1997) ª√—∫ª√ÿߪ√– ‘∑∏‘¿“æ
·≈–°“√· ¥ßÕÕ°¢Õ߬’π∑’Ë àß∂à“¬‡¢â“ Ÿà¢â“«‚¥¬»÷°…“
®“°¬’π‡§√◊ËÕßÀ¡“¬∑’Ë¡’ intron æ∫«à“¬’π∑’Ë¡’ intron ¡’
ª√– ‘∑∏‘¿“æ„π°“√ àß∂à“¬ Ÿß°«à“¬’π∑’ˉ¡à¡’ intron µàÕ
¡“„πªï 1999 Mohanty et al. ª√—∫ª√ÿß°“√ àß∂à“¬¬’π
‡¢â“ Ÿà¢â“«„Àâ¡’ª√– ‘∑∏‘¿“æ Ÿß¢÷Èπ‚¥¬ àß∂à“¬‡¢â“ Ÿàindica
rice æ—π∏ÿå pusa basmati 1 æ∫«à“¡’·§≈≈— ∑’˵â“π∑“π
µàÕ hygromycin B Ÿß∂÷ß√âÕ¬≈– 56 ·≈–¡’®”π«π copy
µ—Èß·µà 1-4 copy ·≈–¡’°“√°√–®“¬µ—«¢Õ߬’π Ÿà√ÿàπ≈Ÿ°
¡’∑—Èß∑’ˇªìπ‰ªµ“¡°Æ¢Õ߇¡π‡¥≈·≈–‰¡à‡ªìπ‰ªµ“¡
°Æ¢Õ߇¡π‡¥≈ πÕ°®“°¢â“«°Áæ∫«à“¡’°“√»÷°…“°“√
àß∂à“¬¬’π‡¢â“ Ÿà°≈⫬‰¡âæ«° phalaenopsis ‚¥¬
Belarmino et al. (2000) ‰¥â»÷°…“ª√– ‘∑∏‘¿“æ
°“√ àß∂à“¬¬’π¢Õß A.tumefaciens 2 “¬æ—π∏ÿå §◊Õ
LBA4404 ·≈– EHA101 ‚¥¬ àß∂à“¬¬’π GUS æ∫«à“
A. tumefaciens ∑—Èß 2 “¬æ—π∏ÿå ¡’ª√– ‘∑∏‘¿“æ„π
°“√ àß∂à“¬¬’π‡∑à“Ê °—π ·≈– Liew et al. (1998)
»÷°…“°“√· ¥ßÕÕ°¢Õ߬’π DFR æ∫«à“„π‡π◊ÈÕ‡¬◊ËÕ∑ÿ°
à«π∑’Ë¡’ ’¡à«ß¢Õß°≈⫬‰¡â¡’°“√· ¥ßÕÕ°¢Õ߬’π DFR
´÷Ë߇°’ˬ«¢âÕß°—∫°“√ —߇§√“–Àå√ß«—µ∂ÿ·Õπ‚∑‰´¬“π‘π
·≈–®“°°“√»÷°…“¢Õß Boase et al. (1998) »÷°…“°“√ àß
∂à“¬¬’π GUS ‡¢â“ Ÿà Chrysanthemum æ—π∏ÿåµà“ß Ê ‚¥¬
A. tumefaciens 4 “¬æ—π∏ÿå ‰¥â·°à EHA105, LBA4404,
MOG101 ·≈– MOG301 ‡«§‡µÕ√å∑’Ë„â àß∂à“¬
§◊Õ pMOG410 ·≈– pKIWI110 ‡æ◊ËÕ‡ª√’¬∫‡∑’¬∫
ª√– ‘∑∏‘¿“æ„π°“√ àß∂à“¬ æ∫«à“ ‡∫≠®¡“»·µà≈–æ—π∏ÿ
°Á‡À¡“–°—∫ A. tumefaciens “¬æ—π∏ÿå∑’˵à“ß°—π
„πß“π«‘®—¬¢—ÈπµàÕ¡“°Á§◊Õ°“√∑¥≈Õß àß∂à“¬¬’π
Õ◊Ëπ∑’Ë π„® (πÕ°‡Àπ◊Õ®“° GUS gene) ‡¢â“ Ÿàæ◊„∫
‡≈’Ȭ߇¥’ˬ« ´÷Ëß°Áæ∫«à“ª√– ∫º≈ ”‡√Á®‡àπ°—π ¥—ß
√“¬ß“π°“√«‘®—¬¢Õß Datta et al. (2000) ∑’Ë»÷°…“°“√
àß∂à“¬¬’π„π indica rice ‚¥¬ A. tumefaciens satrain
LBA4404 (pNO1) ·≈– A281 (pNO1) æ∫«à“
A. tumefaciens ∑—Èß 2 “¬æ—π∏ÿå “¡“√∂ àß∂à“¬ chitinase
gene ‡¢â“ Ÿà embrgogenic calli ¢Õߢ⓫‰¥â ·≈–æ∫
°“√· ¥ßÕÕ°¢Õß chitinase gene ®“°¢â“«∑’Ëæ—≤π“
‡ªìπµâπ‰¥â ®“°°“√ àß∂à“¬¥â«¬ A. tumefaciens ∑—Èß 2
“¬æ—π∏ÿå πÕ°®“°π’È¡’√“¬ß“πª√– ‘∑∏‘¿“æ·≈–§«“¡
”‡√Á®„πæ◊„∫‡≈’Ȭ߇¥’ˬ«Õ◊Ëπ Ê À≈“¬π‘¥ ‡àπ Kamo
(1997) ‰¥â√“¬ß“π°“√ àß∂à“¬¬’π„π Gladiolus æ∫«à“¡’
ª√– ‘∑∏‘¿“æ°“√ àß∂à“¬√âÕ¬≈– 0.5 ®“°°“√»÷°…“°“√
àß∂à“¬¬’π¢Õß Wang and Ge (2005) ´÷Ë߉¥â»÷°…“
°“√ àß∂à“¬¬’π„πæ◊„∫‡≈’Ȭ߇¥’ˬ« Fetuca aundinacea
¥â«¬ A.tumefaciens EHA105 °—∫æ≈“ ¡‘¥
pCAMBIA1305.2 æ∫«à“¡’ª√– ‘∑∏‘¿“æ°“√ àß∂à“¬
¬’π√âÕ¬≈– 1.9 Ledger et al. (1997) àß∂à“¬¬’π„π
lisianthus (Eustoma grandiflorum) æ∫«à“¡’
ª√– ‘∑∏‘¿“æ°“√ àß∂à“¬√âÕ¬≈– 1.9 ‡àπ°—π „π¢≥–∑’Ë
Akasaka-Kennedy, Tomita and Ezura (2004) »÷°…“
°“√ àß∂à“¬¬’π„π Cucumis melo L. æ∫«à“ ¡’
ª√– ‘∑∏‘¿“æ√âÕ¬≈– 2.3 Shrawat et al. (2007)‰¥â»÷°…“
°“√æ—≤π“ª√– ‘∑∏‘¿“æ¢Õß°“√∂à“¬Ω“°¬’π¥â«¬
Agrobacterium tumefaciens ¢Õߢ⓫∫“√å‡≈à¬å ‚¥¬„â
Agrobacterium strain harboring LBA4404 ∑’Ë¡’
binary vector pUGAB7 ´÷Ëߪ√–°Õ∫¥â«¬¬’π bar ·≈–
¬’π uidA À√◊Õ pYF133 ´÷Ëߪ√–°Õ∫¥â«¬¬’π hpt ·≈–
¬’π GFP æ∫°“√ àß∂à“¬¬’π∑’˵â“π∑“πµàÕ bialaphos ·≈–
hygromycin ‚¥¬¡’ª√– ‘∑∏‘¿“æ°“√∂à“¬Ω“°µ—Èß·µà
√âÕ¬≈– 2.6-5.6 ·≈–√âÕ¬≈– 3.3-6.7 µ“¡≈”¥—∫