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3 Media 3.25 Medien Ham‘s F12 Medium/Ham‘s F12 Medium Schedule/Tabelle L-Glutamine Stab. Glutamine Sodium pyruvate Phenol red Hepes Special 14550 X X 14500 X X X 14501 X 25 mM 15500 X X X 14559 X 15600 X X X See above / s.o. Each formulation possible/Jede abweichende Formulierung möglich BIOTECH GmbH
Media Ham‘s F10 is an alternative of Ham‘s F12 and was used primarily to culture CHO-cells. Today Ham‘s F10 can be used with or without FBS for many different cell cultures. It finds a use for example in primary cells of rat and chicken, but also for human diploid cells. Composition/Zusammensetzung BIOTECH GmbH Components mg/L Inorganic Calcium chloride x 2H2O 44,09 Salts Copper(II) sulfate x 5H2O 0,003 Iron(II) sulfate x 7H2O 0,834 Magnesium sulfate dried 74,62 Potassium chloride 285,00 Potassium dihydrogen 83,00 phosphate Sodium chloride 7400,00 di-Sodium hydrogen phosphate 153,70 anhydrous Zinc sulfate x 7H2O 0,029 Other D(+)-Glucose anhydrous 1100,00 Compo- Hypoxanthine 4,08 nents DL-α-Lipoic acid 0,21 Hepes 5958,00 Phenol red 1,20 Sodium pyruvate 110,00 2`-Deoxythymidine 0,73 Amino L-Alanine 8,91 acids L-Arginine x HCl 211,00 L-Asparagine x H2O 15,00 L-Aspartic acid 13,30 L-Cysteine x HCl x H2O 35,12 L-Glutamine 146,20 L-Glutamic acid 14,70 Glycine 7,51 L-Histidine x HCl x H2O 21,00 L-Isoleucine 2,60 L-Leucine 13,10 L-Lysine x HCl 29,30 L-Methionine 4,48 L-Phenylalanine 4,96 L-Proline 11,50 L-Serine 10,50 L-Threonine 3,57 L-Tryptophan 0,60 L-Tyrosine 1,81 L-Valine 3,50 Vitamins D(+)-Biotin 0,024 D-Calcium pantothenate 0,715 Choline chloride 0,698 Folic acid 1,32 myo-Inositol 0,541 Nicotinamide 0,615 Pyridoxine x HCl 0,21 Riboflavin 0,376 Thiamine x HCl 1,01 Vitamin B12 1,36 When 5958,00 mg/l Hepes are included there are only 6900 mg /l Sodium chloride. Wenn 5958,00 mg/l Hepes enthalten sind, sind nur 6900 mg /l Natriumchlorid enthalten. 3.26 Medien Ham‘s F10 Medium/Ham‘s F10 Medium Description/Beschreibung Ham‘s F10 als Alternative zum Ham‘s F12 diente in der Vergangenheit vorrangig der Kultivierung von CHO-Zellen. Heute wird das Ham‘s F10 in verschiedenen Kulturen mit oder ohne Kälberserum eingesetzt. Verwendung findet es zum Beispiel bei primären Zellen von Ratte und Huhn, aber auch bei humanen Diploidzellen. Liquid Media/Flüssigmedien Ham's F10 Medium 500 ml P04-12050 without L-Glutamine with 1,2 g/l NaHCO3 Ham’s F10 Medium 500 ml P04-12500 with L-Glutamine with 1,2 g/l NaHCO3 Ham’s F10 Medium 500 ml P04-13500 with stab. Glutamine with 1,2 g/l NaHCO3 Ham’s F10 Medium 500 ml P04-13050 with L-Glutamine with 25 mM Hepes with 1,2 g/l NaHCO3 Special Media/Spezialmedien Minimum order quantity: 20 x 500 ml/ Mindestabnahmemenge: 20 x 500 ml Ham’s F10 Medium 500 ml P04-12049 without L-Glutamine without Phenol red with 1,2 g/l NaHCO3 Ham’s F10 Medium 500 ml P04-13501 with stab. Glutamine without Glucose with 1,2 g/l NaHCO3 Each formulation possible/Jede abweichende Formulierung möglich 3
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Serum-free Systems Serum-freie Syst
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Serum-free Systems The Serumfree Ce
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Serum-free Systems Detailed informa
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Serum-free Systems PANEXIN NTA is a
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Serum-free Systems Cells Count at d
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Serum-free Systems Adaptation instr
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Serum-free Systems Panexin BMM is a
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Serum-free Systems In adherent cell
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Serum-free Systems Panserin 604 is
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Serum-free Systems Panserin 293S is
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Serum-free Systems BIOTECH GmbH SP2
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Serum-free Systems BIOTECH GmbH CHO
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Serum-free Systems Instructions for
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Serum-free Systems Panserin ProVero
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Serum-free Systems Indirect adaptat
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Serum-free Systems Instructions for
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Serum-free Systems Special Advantag
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Serum-free Systems Panserin 411S is
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Serum-free Systems Production and s
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Serum-free Systems Panserin 604SPF
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Serum-free Systems Typical Growth C
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Serum-free Systems Preparation of P
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Serum-free Systems Preparation of P
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Page 87 and 88: Cell Culture Media Zellkulturmedien
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Page 123 and 124: Media McCoy’s 5A Medium (modified
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Page 131 and 132: Media MEM Eagle with EBSS 500 ml P0
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Page 147 and 148: Media Background: Endothelial cells
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Page 151 and 152: Media Stem cells are non-specialize
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Reagents BIOTECH GmbH 4.8 Reagenzi
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Reagents Acid-soluble collagen from
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Reagents Albumins conduct as a prot
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Reagents Ready-to-use tubes Benefit
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Reagents BIOTECH GmbH 4.16 Reagenz
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Services Dienstleistungen Introduct
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Services Protein Colorimetric test
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Services Plating efficiency To get
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Biologicals Biologika Introduction
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Biologicals BIOTECH GmbH 6.2 Biolo
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Biologicals BIOTECH GmbH 6.10 Biol
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Molecular Biology Molekularbiologie
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Molecular Biology Features and appl
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Molecular Biology Reagents Specific
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Molecular Biology BIOTECH GmbH 7.1
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Molecular Biology Features • 14 b
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Molecular Biology Features • 12 b
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Laboratory Materials Labormateriali
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Laboratory Materials Mycoplasma Det
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Laboratory Materials Ready for use
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Laboratory Materials Disinfectant c
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Laboratory Materials Application Pr
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Annex Anhang General Terms and Cond
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Annex Dangers Some of our substance
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Annex BIOTECH GmbH 9.4 Anhang Cust
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Annex BIOTECH GmbH 9.6 Anhang Prod
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Numeric Product Index Art-No./ Page
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