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7 Molecular Biology 7.23 Molekularbiologie Additives and Buffer/Additive und Puffer Features and applications • Eliminates background smears and spurious bands • Improves specificity • Compatible with all commercially available DNA polymerases • Ideal for difficult templates • Improving the specificity of any DNA polymerase in enzyme reactions HiSpec additive is a popular compound designed to eliminate unwanted byproducts, such as background smears and spurious bands, during DNA amplification. Hi-Spec Additive is ideally suited to difficult templates with GC-rich regions or repetitive sequences. Storage Conditions HiSpec additive can be stored for 6 months at -20° C. HiSpec Additive/HiSpec Additiv Description/Beschreibung HiSpec additive 3 x 1,2 ml PAN737032 BIOTECH GmbH
Molecular Biology BIOTECH GmbH 7.24 Molekularbiologie Additives and Buffer/Additive und Puffer Features and applications • Dramatically improves specificity and yield • Compatible with all commercially available thermostable DNA polymerases • Ideal for difficult templates • Reduces smearing and background • DNA Polymerase reactions where specificity is critical • Enhancing the performance and specificity of any thermostable DNA polymerase PAN Mate is a special 2x additive for use in reactions involving any thermostable DNA polymerase, and is designed to dramatically improve reaction specificity. PAN Mate provides an optimized composition of reagents, and is ideally suited to dirty/difficult templates with GC or AT rich DNA, repetitive sequences or sequences with a high level of secondary structure. PAN Mate acts as a melting agent by allowing the DNA polymerase and oligonucleotides greater access to the template DNA. PAN Mate does not contain magnesium, dNTPs, or buffer components. In some cases it may be necessary to optimize the Magnesium concentration. Note: PAN Mate should not be used in combination with any other additives for polymerase reactions. PAN Mate is a revised version of PAN 5x HiSpec Additive (PAN737032). Storage Conditions PAN Mate additive can be stored for 6 months at -20° C. PCR of a 201 bp GC-Rich fragment > 66 % from Human TGF-8 gene Lane 1 - 3: With PAN Mate & 1.5 mM, 2.0 mM & 2.5 mM MgCl 2 respectively Lane 4: PANLadder IV Lane 5 - 7: Without PAN Mate or MgCl2 PAN Mate Additive/PAN Mate Additiv Description/Beschreibung PAN Mate assayed with three different polymerases on a 234 bp GC-Rich fragment > 66 % from Human ApoE gene Lane 1: PANLadder II Lane 2: Treated with PAN Mate Lane 3: Without PAN Mate PAN Mate assayed with three different polymerases on a 201 bp GC-Rich fragment > 66 % from Human TGF-b gene Lane 1: PANLadder II Lane 2: Treated with PAN Mate Lane 3: Without PAN Mate PAN Mate Additive 2 x 1,2 ml PAN737041 7
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Serum-free Systems Serum-freie Syst
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Serum-free Systems The Serumfree Ce
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Serum-free Systems Detailed informa
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Serum-free Systems PANEXIN NTA is a
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Serum-free Systems Panexin BMM is a
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Serum-free Systems In adherent cell
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Serum-free Systems Panserin 604 is
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Serum-free Systems Panserin 293S is
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Serum-free Systems BIOTECH GmbH SP2
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Serum-free Systems Instructions for
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Serum-free Systems Panserin ProVero
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Sera METHOD: Serum is heated for 30
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Sera Other Animal Sera/Andere Tiers
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Sera Human Serum is manufactured fr
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Cell Culture Media Zellkulturmedien
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Media Alpha MEM is a different form
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Media 21050 21060 X BIOTECH GmbH 3
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Media Composition/Zusammensetzung 3
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Media Microbiological Medium for th
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Media BIOTECH GmbH 3.10 Medien Dul
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Media BIOTECH GmbH 3.20 Medien DME
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Media The Grace‘s insect medium w
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Media Ham‘s F10 is an alternative
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Media BIOTECH GmbH 3.28 Medien Isc
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Media Modification of MEM for suspe
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Media Leibovitz’s L-15 Medium 10
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Media McCoy’s 5A Medium (modified
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Media BIOTECH GmbH 3.38 Medien MCD
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Media MEM Eagle with EBSS 500 ml P0
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Media BIOTECH GmbH 3.46 Medien MEM
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Media The medium was developed for
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Media BIOTECH GmbH 3.52 Medien Sch
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Media The TNM-FH is a variation of
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Media The William‘s medium E is u
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Media 3.58 Medien William‘s Mediu
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Media Background: Endothelial cells
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Media Melanozytes are embedded in t
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Media Stem cells are non-specialize
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Media For bone marrow cells in tumo
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Reagents Reagenzien Media Supplemen
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Reagents BIOTECH GmbH 4.2 Reagenzi
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Reagents Composition/Zusammensetzun
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Reagents Acid-soluble collagen from
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Reagents Albumins conduct as a prot
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Reagents Ready-to-use tubes Benefit
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Reagents BIOTECH GmbH 4.16 Reagenz
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Services Dienstleistungen Introduct
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Page 179 and 180: Biologicals Biologika Introduction
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