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3 Media L-15 contains no sodium hydrogen carbonate and no bicarbonate, because it is buffered already by the high concentration of amino acids. The L-15 medium supports the growth of established cells like Hep-2, but also human nerve cells and primary tissue explantates. With 10 % tryptose phosphate broth it is also ideally suitable for the cultivation of insect cell lines. Composition/Zusammensetzung 3.33 Medien Leibovitz‘s L-15 Medium/Leibovitz‘s L-15 Medium Components mg/L Inorganic Calcium chloride x 2H2O 185,44 Salts Magnesium chloride x 6H2O 200,00 Magnesium sulfate dried 139,52 Potassium chloride 400,00 Potassium dihydrogen 60,00 phosphate Sodium chloride 8000,00 di-Sodium hydrogen phosphate 190,00 Other D(+)-Galactose anhydrous 900,00 Compo- Hepes 5958,00 nents Phenol red 10,00 Sodium pyruvate 550,00 Amino L-Alanine 225,00 acids L-Arginine Base 500,00 L-Asparagine x H2O 250,00 L-Cysteine 120,00 L-Glutamine 300,00 Glycine 200,00 L-Histidine Base 250,00 L-Isoleucine 250,00 L-Leucine 125,00 L-Lysine x HCl 93,75 L-Methionine 75,00 L-Phenylalanine 125,00 L-Serine 200,00 L-Threonine 300,00 L-Tryptophan 20,00 L-Tyrosine 300,00 L-Valine 100,00 Vitamins D-Calcium pantothenate 1,00 Choline chloride 1,00 Folic acid 1,00 myo-Inositol 2,00 Nicotinamide 1,00 Pyridoxol x HCl 1,00 Riboflavin-5’-phosphate sodium 0,1075 salt x H2O Thiamine monophosphate 1,00 chloride When 5958,00 mg/l Hepes are included there are only 7500,00 mg /l Sodium chloride. Wenn 5958,00 mg/l Hepes enthalten sind, sind nur 7500,00 mg /l Natriumchlorid enthalten. Description/Beschreibung L-15 enthält kein Natriumhydrogencarbonat, da es durch den hohen Gehalt an Aminosäuren schon gepuffert wird. Das Medium unterstützt das Wachstum von etablierten Zelllinien wie zum Beispiel Hep-2, wie auch humaner Nervenzellen und primärer Gewebeexplantate. Mit 10 % Tryptose-Phosphat-Broth ist es außerdem sehr gut für die Kultivierung von Insektenzelllinien geeignet. Liquid Media/Flüssigmedien Leibovitz’s L-15 Medium 500 ml P04-27055 without L-Glutamine without NaHCO3 Leibovitz’s L-15 Medium 500 ml P04-27500 with L-Glutamine without NaHCO3 Leibovitz’s L-15 Medium 500 ml P04-27050 with stab. Glutamine without NaHCO3 Special Media/Spezialmedien Minimum order quantity: 20 x 500 ml/ Mindestabnahmemenge: 20 x 500 ml Leibovitz’s L-15 Medium 500 ml P04-27053 with L-Glutamine with 2,0 g/l NaHCO3 Leibovitz’s L-15 Medium 500 ml P04-27054 without L-Glutamine without Phenol red without NaHCO3 Each formulation possible/Jede abweichende Formulierung möglich BIOTECH GmbH
Media Leibovitz’s L-15 Medium 10 L P03-1510 with L-Glutamine 50 L P03-1550 without NaHCO3 BIOTECH GmbH 3.34 Medien Leibovitz‘s L-15 Medium/Leibovitz‘s L-15 Medium Powder Media/Pulvermedien Schedule/Tabelle Leibovitz’s L-15 Medium 10 L P03-1610 with L-Glutamine 50 L P03-1650 with 25 mM Hepes without NaHCO3 L-Glutamine Stab. Glutamine Sodium pyruvate Phenol red Special 27055 X X 27500 X X X 27050 X X X 27053 X X X See above / s.o. 27054 X See above / s.o. Each formulation possible/Jede abweichende Formulierung möglich 3
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Serum-free Systems In contrast, it
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Serum-free Systems Sub-confluent hM
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Page 87 and 88: Cell Culture Media Zellkulturmedien
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Page 109 and 110: Media The Grace‘s insect medium w
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Page 131 and 132: Media MEM Eagle with EBSS 500 ml P0
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Page 147 and 148: Media Background: Endothelial cells
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Reagents BIOTECH GmbH 4.16 Reagenz
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Services Dienstleistungen Introduct
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Services Protein Colorimetric test
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Services Plating efficiency To get
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Biologicals Biologika Introduction
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Biologicals BIOTECH GmbH 6.2 Biolo
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Molecular Biology Molekularbiologie
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Molecular Biology Features and appl
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Molecular Biology Reagents Specific
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Molecular Biology BIOTECH GmbH 7.1
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Molecular Biology Features • 14 b
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Molecular Biology Features • 12 b
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Laboratory Materials Labormateriali
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Laboratory Materials Mycoplasma Det
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Laboratory Materials Disinfectant c
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Laboratory Materials Application Pr
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Annex Anhang General Terms and Cond
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Annex Dangers Some of our substance
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Annex BIOTECH GmbH 9.4 Anhang Cust
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