BUKU ABSTRAK - Universiti Putra Malaysia

Mesenchymal Stem Cells Modulate Neutrophils’ Respiratory Burst Activity: An
Impact on Chronic Inflammatory Diseases
Dr. Rajesh Ramasamy
Maryam Maqbool and Sharmili Vidyadaran
Faculty of Medicine and Health Sciences, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2377; r.rajesh@medic.upm.edu.my
Neutrophils or polymorphonuclear leucocytes (PMN) are major cell type that constitutes innate immunity.
They comprise approximately 50-70% of leucocytes and predominate in eliminating pathogens that induce acute
inflammation. In bone marrow, mesenchymal stem cells (MSC) protect neutrophils from apoptosis, preserving
their effector functions and preventing excessive or inappropriate activation of the oxidative burst. PMN use
oxygen-dependent mechanisms that involve the production of reactive oxygen species (ROS) and reactive
nitrogen species (RNS). Generation of ROS from stimulated neutrophils is thought to play an important role
in host defence and tissue damage. Therefore the current study, we evaluate the effects of MSC on neutrophils
respiratory burst activity. Chemiluminescence and Griess assays are widely used as sensitive and accurate
measurements to assess the neutrophils’ reactive oxygen and nitrogen species production. The effect of MSC on
generation of ROS by neutrophils was evaluated at different time points in the present of absence of MSC. Our
study shows that MSC at all ratios in 24 and 48 hours inhibit the ROS production in chemiluminescence assay.
Furthermore, in Griess assay, it was observed that at 24 and 48 hours, MSC inhibit the nitric oxide production.
Our results indicate that MSC could be potentially serve as therapy to dampen neutrophils respiratory burst during
an exaggerated neutrophils responses whereby the over production of ROS and RNS can cause harmful effects
to other neighbouring cells and tissue. MSC can be also proposed as a component in treating chronic neutrophils
responses such as arthritis and other chronic inflammatory diseases.
Keywords: Neutrophil, respiratory burst, mesenchymal stem cells
Anti-inflammatory and Anti-pyretic Effects of Hexane Fraction of Ardisia crispa
Dr. Roslida Abd. Hamid
Lau Moi Fong, Sabrina Sukardi and Muhammad Nazrul Hakim Abdullah
Faculty of Medicine and Health Sciences, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2341; roslida@medic.upm.edu.my
Hexane fraction of Ardisia crispa root (ACHE) was used to investigate its anti-inflammatory and anti-pyretic
activities in this study. For anti-inflammatory activity, 12-O-tetradecanoylphorbol-13-acetate (TPA) was applied
to the ear of mice to induce oedema and treated with 0.5,1 and 2mg/ear of ACHE topically. In cotton-pellet
granuloma test, treated groups have received 3, 10, 30 and 100mg/kg of hexane extract administered orally for
seven days. For antipyretic activity, brewer’s yeast was injected in mice to induce fever and later, ACHE at dose
ranging from 10 to 300 mg/kg was administered to the rats orally. The results exhibited that 1 and 2mg/ear of
ACHE produced significant suppression by 19.9% and 20.2% respectively. The lowest dose of ACHE showed no
significant effect when compared with control. Results showed that ACHE showed significant anti-pyretic effect
at all doses (10, 30, 100 and 300 mg/kg). At 30, 100 and 300mg/kg, ACHE even exhibited higher efficacy when
compared with 100 mg/kg acetaminophen. ACHE also elicited a significant (P