BUKU ABSTRAK - Universiti Putra Malaysia

Recombinant Vectors for Cancer Gene Therapy
Dr. Zeenathul Nazariah Allaudin
Tan Seok Shin and Mohd. Azmi Mohd. Lila
Faculty of Veterinary Medicine, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 8055; zeenathul@putra.upm.edu.my
Non replicate viral based vector expressed of a novel poultry gene is able to induced apoptosis in cancer cells.
Besides, non replicate viral based vector expressed of murine endostatin gene is able to induced anti-angiogenesis
effect in tumour mass. The objective of this study is to investigate the apoptosis and anti-angiogenesis effect of
the poultry and endostatin gene respectively in cancer cells. DNA fragmentation test, TUNEL assay and FITC
Annexin V/ PI double staining test were carried out as apoptosis tests. In addition, casapses test were also used to
investigate the apoptosis pathway induced by recombinant adenovirus. Tumour mass growth rates in mice were
studied as a model for the effectiveness of both constructs in cancer treatment. All results showed positive in the
use of recombinant adenovirus as anti-cancer vaccine. This research warrants greater insight to the anti-cancerous
potential of the recombinant adenovirus constructs.
Keywords: Recombinant adenovirus, apoptosis, cancer cells, tumour mass, anti-cancerous
Effect of Momordica charantia on Histopathological Changes associated with STZ-
Induced Diabetes in Neonatal Rats
Mr. Md. Zuki Abu Bakar@Zakaria
Noordin Mohamed Mustapha and Goh Yong Meng
Faculty of Veterinary Medicine, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 8301; zuki@vet.upm.edu.my
The study was conducted to determine the effects of Momordica charantia (MC) on pancreas, liver and
renal histopathological changes in STZ-induced diabetes in neonatal rats. Diabetes mellitus was induced in 28
day-old Sprague-Dawley neonatal rats using single intrapretoneal injection of STZ (85 mg/kg body weight).
The animals were divided into four groups: nSTZ/16 (diabetic control), nSTZ/M group (fruit aqueous extract),
nSTZ/G group (glibenclamide), and NC16 (non-diabetic). Biochemical and histological evaluations were carried
out before treatment and four weeks post-treatment. The results showed significant decrease (P