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BUKU ABSTRAK - Universiti Putra Malaysia

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Forestry & Environment<br />

Chemometric Approach to Validating Faecal Sterols as Source Tracer for Faecal<br />

Contamination in Water from Langat River, <strong>Malaysia</strong><br />

Dr. Hafizan Juahir<br />

Nur Hazirah Adnan, Munirah Abdul Zali, Nurul Afiqah Mohamd Tahir, Norashikin Saim, Rozita Osman,<br />

Dayang Ratena Sari Abg. Spian, Mohd. Zuli Jaafar, Md. Pauzi Abdullah and Fuzziawati Ab. Ghani<br />

Faculty of Environmental Studies, University <strong>Putra</strong> <strong>Malaysia</strong>,<br />

43400 UPM Serdang, Selangor, <strong>Malaysia</strong>.<br />

+603-8946 7460; hafizan@env.upm.edu.my<br />

The municipal and anthropogenic activities have been verified as substantial contributors in the Langat River<br />

Basin. Faecal sterols fingerprinting method is a promising method for sources classifications of faecal pollutant<br />

due to its continuous discharge into the riverine ecosystem. In this study, faecal contamination in water samples<br />

from point source (sewage treatment plants, chicken farms, quail farms and horse stables) was extracted using the<br />

solid phase extraction (SPE) technique and analyzed using Gas Chromatography- Mass Spectrometry (GC-MS).<br />

Faecal sterols (coprostanol, cholesterol, stigmasterol? -sitosterol and stigmastanol) were selected as parameters to<br />

differentiate the source of faecal pollution. The results indicated that coprostanol, cholesterol and?-sitosterol were<br />

the most significant parameters that can be used as source tracers for faecal pollution. Chemometric techniques,<br />

such as cluster analysis (CA), principal component analysis (PCA) and discriminant analysis (DA) were applied<br />

to the data set on faecal contamination in water from various pollution sources in order to validate the faecal<br />

sterols’ profiles. CA generated three clusters: coprostanol was in cluster 1, cholesterol and? -sitosterol formed<br />

cluster 2, while cluster 3 contained stigmasterol and stigmastanol. DA suggested that coprostanol, cholesterol<br />

and? -sitosterols were the most significant parameters to discriminate between the faecal pollution sources.<br />

Applications of chemometric techniques revealed beneficial and potential methods in discovering the source of<br />

faecal contamination in marine environment.<br />

Keywords: Sterols, faecal contamination, solid phase extraction, principal component analysis, cluster analysis<br />

A General Model for Estimating Tropical Forest Biomass<br />

Dr. Hazandy Abdul Hamid<br />

Ika Heriansyah, Nor Aini Ab. Shukor, and Syamsudin Ibrahim<br />

Faculty of Forestry, University <strong>Putra</strong> <strong>Malaysia</strong>,<br />

43400 UPM Serdang, Selangor, <strong>Malaysia</strong>.<br />

+603-8946 7585; hazandy@gmail.com<br />

Attempts to estimate the biomass density of tropical forests have been made by the scientific community<br />

for use in models that assess the contribution of tropical deforestation and biomass burning to the increase in<br />

atmospheric carbon dioxide and other trace gases. However, because so many different equations and techniques<br />

are needed to estimate various structures and compositions of tropical forest and it would be a huge and costly,<br />

improving biomass estimates by constructing new equations to present all tree species in all locations are necessary.<br />

The main goals of this study are to present simple methods that are available for estimating biomass density of<br />

tropical forests or tree formations using primarily existing data. Prediction equations for biomass were generated<br />

from easily measurable parameters such as diameter at breast height and total height. Data on dry weight of all<br />

biomass components of sample trees were used to develop prediction equations. The power equations were used<br />

for biomass equation because these equations had proved superior to other equations as evident from its greater<br />

values of coefficient of determination as well as from lesser values of standard error for most of the biomass<br />

components. Equations were presented from destructive sampling of 250 sample trees of 19 species in Indonesia,<br />

Papua New Guinea and Vietnam, from 1.20 cm to 109.18 cm of D and from 1.9 m to 57.7 m of H. The study<br />

provided two steps of biomass estimation, i.e., (1) a multi step process that entails calculating volume and then<br />

converting volume to dry weight biomass and (2) a one-step process that calculate biomass directly from growth<br />

parameter of D or combination D2H. Biomass density can be calculated from volume/ha by first estimating the<br />

biomass of the inventoried volume and then “expanding” this value to take into account the total biomass.<br />

Keywords: Diameter, general equation, estimator, biomass, tropics<br />

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