BUKU ABSTRAK - Universiti Putra Malaysia

Myoblast Seeded Bovine Pericardium for Repair of Abdominal Wall Defect
Mr. Md. Zuki Abu Bakar@Zakaria
Ayele Taddese Tsedeke, Noordin Mohamed Mustapha and Noorjahan Banu Mohamed Alitheen
Faculty of Veterinary Medicine, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 8301; zuki@vet.upm.edu.my
Keywords: Tissue engineering, muscle, bovine pericardium, abdominal wall
Myoblast Seeded Bovine Tunica vaginalis for Repair of Abdominal Wall Defect
Mr. Md. Zuki Abu Bakar@Zakaria
Ayele Taddese Tsedeke, Noordin Mohamed Mustapha and Noorjahan Banu Mohamed Alitheen
Faculty of Veterinary Medicine, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 8301; zuki@vet.upm.edu.my
Keywords: Tissue engineering, muscle, bovine tunica vaginalis, abdominal wall defect, cell-seeding
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Science, Technology & Engineering
Abdominal wall defects caused by trauma, tumour ablation, and post-surgical loss of muscle mass may
lead to loss of functional muscle tissue and cause severe impairments of the functionality of skeletal muscle.
Tissue engineering is a promising approach to replace the lost or defective muscle tissue. The aim of this study
was to engineer skeletal muscle tissue for reconstruction of abdominal wall defects in a rabbit model. Myoblast
were harvested successfully from soleus muscles of 5-day-old male White New Zealand rabbit and based on
the purity test using immunocytochemistry and flow cytometric analysis, more than 97% of the isolated skeletal
myoblast have got myogenic phenotype. PKH26-fluorescent labelled myoblast were seeded onto the scaffolds and
incubated in vitro for 5 days. Full thickness abdominal wall defects (3 x 4 cm) were created in 18 anaesthetised
male New Zealand white rabbits and randomly divided into two equal group (n=9 each). The defects of the
first group were repaired with myoblast seeded bovine pericardium while the second group with non-seeded
bovine pericardium (control group). Three animals were sacrificed at 7, 14, and 30 days after implant from
each group and the explanted specimens were subjected to macroscopic, and light, fluorescence and electron
microscopic analysis. Mild adhesions were detected in control group, while they were not present in the treatment
experiment implants group. In every case, tissue engineered construct was thicker in deposition of newly formed
collagen with neo vascularisation than controls. Multinucleated myotubes and myofibres were only detected in
the treatment experiment group. Therefore, engineered soft tissue construct can provide structural replacement of
severe and large abdominal wall defects.
The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast
were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3x4 cm)
were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of
the first group were repaired with myoblast-seeded-bovine Tunica vaginalis whereas the second group repaired
with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at seventh,
fourteenth, and thirtieth of post-implantation from each group and the explanted specimens were subjected to
macroscopic and microscopic analysis. In every case, seeded-scaffolds have better deposition of newly formed
collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibres were
only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine Tunica vaginalis can
be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal
muscle tissue.