BUKU ABSTRAK - Universiti Putra Malaysia

Agriculture
Assessment of Growth Condition for a Candidate Probiotic, Shewanella algae,
Isolated from the Digestive System of a Healthy Juvenile Penaeus monodon
Assoc. Prof. Dr. Che Roos Saad
SaadShahram Shakiba Zadeh, Annie Christianus, Mohd. Salleh Kamarudin, Kamaruzaman Sijam, Mariana Nor
Shamsudin and Vasantha Kumari Neela
Faculty of Agriculture, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+608-946 4109; cheroos@agri.upm.edu.my
To combat disease problem in shrimp industries, probiotic biocontrol is a well known remedy now. The
antagonistic ability of separated isolates from different parts of juvenile P. monodon was screened against shrimp
Vibrio pathogens, V. parahaemolyticus and V. alginolyticus. The most antagonistic effect was observed for an
isolate that primarily identified as ShewDiversity and Beneficial Endophytes from Mangosteen and Brunei Cherry
anella algae using conventional method followed by Biolog GN and GP microplates. Since adaptability to the
host optimum cultural condition of the target organism is of the great importance, response surface methodology,
with central composite design, was applied to assess log cell count response of S. algae in different incubation
conditions. Therefore, four independent variables were assumed as: temperature (10- 50C), pH (6-10), NaCl
concentration (0-50%) and time (12-60 h). The coefficients of multiple determinations (R2) for the responses log
cell count of S. algae being 0.827. Temperature was the merely significant independent variable that affected the
log cell count of the candidate probiotic. The candidate probiotic revealed a reasonable growth response in quite
wide range of temperature, pH and NaCl concentration in which the maximum levels were in the same range of
optimum shrimp culture.
Keywords: Bacterial growth, penaeus monodon, probiotic, s algae, V. parahaemolyticus, v. alginolyticus
Diversity and Beneficial Endophytes from Mangosteen and Brunei Cherry
Assoc. Prof. Dr. Cheah Yoke Kqueen
Sim Jiun Horng, Khoo Chai Hoon and Son Radu
Institute of Bioscience, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2343; ykcheah@medic.upm.edu.my
Garcinia is commonly found in Malaysia, but limited information is available regarding endophytic fungi
associated with this plant. In this study, 24 endophytic fungi were successfully recovered from different parts
of two Garcinia species. Characterisation of endophytic fungi was performed based on the conserved internal
transcribed spacer (ITS) region sequence analysis and the antimicrobial properties. Results revealed that fruits of
the plant appeared to be the highest inhabitation site (38%) as compared with others. Glomerella sp., Guignardia
sp., and Phomopsis sp. appeared to be the predominant endophytic fungi group in Garcinia mangostana and
Garcinia parvifolia. Phylogenetic relationships of the isolated endophytic fungi were estimated from the
sequences of the ITS region. On the other hand, antibacterial screening showed 11 of the isolates possessed
positive response towards pathogenic and nonpathogenic bacteria.
Keywords: Mangosteen, Brunei Cherry, enophytic fungi, antimicrobial
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