BUKU ABSTRAK - Universiti Putra Malaysia

Health
Invention/Innovation Title: The Suppression Mechanism of Rice Bran Phytic Acid
in In Vivo and In Vitro Studies as Anticancer Agent
Mrs. Norazalina Saad
Norhaizan Mohd. Esa, Sabariah Abdul Rahman, Hairuszah Ithnin and Nurul Husna Shafei
Institute of Bioscience, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2212; norazalina@putra.upm.edu.my
A striking anticancer action of phytic acid has been demonstrated both in vivo an in vitro in a variety of
tumour types, possibly via inhibition of tumour cell growth and differentiation. Towards the present research
finding, phytic acid extracted from rice bran showed inhibitory effects in in vitro and in vivo analysis. Regarding
to the microculture 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that
phytic acid has the highest sensitivity towards colon cancer cell (HT29) with IC50 values of 12.0 ± 1.21 ?g/
ml followed by CaOV-3 (human ovary cancer cells), MCF-7 (human breast cancer cells), HepG2 (human liver
cancer cells) with IC50 values 22.0 ± 2.23 ?g/ml, 32.0 ± 2.56 ?g/ml, 33.0 ± 3.21 ?g/ml respectively. Analysis
of Annexin V-FITC/Propidium Iodide revealed that phytic acid induced apoptosis in HT29. In animal study,
rats received two intraperitoneal injections of azoxymethane (AOM) in saline at (15mg/kg bodyweight) over
a two-week period to induce colonic cancer. The finding showed treatment with phytic acid gave the reduction
in the formation of aberrant crypt foci (ACF) after eight weeks and significantly suppressed the number of
ACF in the distal, middle and proximal colon as compared to AOM alone (p