BUKU ABSTRAK - Universiti Putra Malaysia

Artificial Conduit for Coronary Heart Diseases
Dr. Siti Aslina Hussain
Tan Hong Tat, Mohd. Ismail Abdul Halim, Norhafizah Abdullah and Azni Idris
Faculty of Engineering, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 6292; aslina@eng.upm.edu.my
A classical treatment to heart attack is usually done by undergoing a bypass surgery where the bypass grafts
are frequently harvested from internal thoracic arteries, radial arteries or saphenous veins known as coronary
sinus bypass surgery. The vein is usually harvested from the leg or stomach of human. In order to prevent the risk
of patency and infections, an artificial coronary sinus conduit was produced throughout numerical studies of blood
flow system of aorta coronary sinus conduit. The model was carried out using ANSYS CFD (CFX 12.2 codes)
simulation. Pressure drop was investigated to achieve from 80mmHg to 15mmHg with a 3D model blood conduit.
It is found that a narrow tube needs to be incorporated into the conduit model. This is to induce a venturi effect in
order to reduce the pressure of blood within a specific throat length. A model of 3mm inlet and throat diameter of
1.13mm throat diameter shows the best result for pressure reduction from 80mmHg to 15mmHg. This model also
has a lower peak velocity (laminar flow), which is more preferable in blood flow in vein.
Keywords: Coronary sinus, artificial, CFD, simulation, heart diseases
Adaptability of MRSA to Different Host: A Proteomic Approach
Dr. Vasanthakumari Neela
Liew Yun Khoon, Mariana Nor Shamsudin, Rukman Awang Hama and Chong Pei Pei
Faculty of Medicine and Health Sciences, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2507; neela2000@hotmail.com
In our earlier study, we found that MRSA-ST9-t4358-SCCmec V colonizes pigs (1.38%) and pig handlers
(5.5%) in Malaysia. In facing the new emerging clonal type MRSA colonization among humans from pigs, we
were driven to perform the fundamental investigation on MRSA-ST9 exoproteome to answer the most basic
scientific question, whether the expression of exoprotein by same clonal type isolates origin from different host
are highly variable. Hence, the total of six isolates belonging to MRSA-ST9 in this study was selected (divided in
two groups equally: pig isolates and pig handler isolates). Mid-exponential phase of exoprotein from each isolates
was extracted, precipitated and partial purified by ultracentrifuge and TCA-ethanol method. Two-dimensional
gel electrophoresis (2DGE) was applied to the purified protein and the selected protein spot was identified by
MALDI-TOF/TOF analysis. PDquest software was concomitantly carried out with total density in gel image
normalization method associated with parts per million (PPM) as a scaling factor. Minor variability was observed
in the exoprotein patterns between pig isolates and pig handler isolates. In addition, high degree of similarity
in exoprotein patterns among the intra-individual in each group was also revealed using the 2DGE comparison
data. Nevertheless, the immunodominant staphylococcal antigen A (IsaA) was only found to be produced in all
of the pig isolates during mid-exponential growth phase. Hence, questions were raised whether IsaA is expressed
in low quantity by pig handler isolates or IsaA gene is controlled by different novel transcriptional regulatory
system? Overall, 2-DGE analysis of MRSA-ST9 exoprotein suggests that there are no major variations in terms
of exoprotein production and regulation, between same clonal lineages isolates. However, whether the efficiency
of virulence factor of MRSA-ST9 will be steadily maintained even though transmission of different host system
occurred over a time period is not clear.
Keywords: Exoprotein, staphylococcus aureus, immunodominant staphylococcal antigen A
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Health