BUKU ABSTRAK - Universiti Putra Malaysia

Bonigen: A Potential Anti-biofilm Bone Substitute
Prof. Dr. Fauziah Othman
Au Lee Fong, Sharmili Vidyadaran, Asmah Rahmat, Rusnah Mustaffa, Idris Besar, Azfar Rizal Ahmad, Fuzina
Nor Hussein, Mohd. Azam Khan Goriman Khan, Abdah Md. Akim, Saidi Moin and Che Nor Zarida Che Seman
Faculty of Medicine and Health Sciences, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2315; fauziah@putra.upm.edu.my
Biomaterials have been widely used in the tissue engineering of bone as medical devices. It plays important
role in treating diseases and improving healthcare, which are commonly used in dental, surgery and drug delivery
applications. Hydroxyapatite (HA) is the major inorganic component of hard tissue in human body and is also
known to have superior biocompatibility and can be reabsorbed and replaced by bone to repair the bone damage
zone. Therefore, HA loaded with suitable antibiotics can be used as a carrier to limit biofilm formation. Biofilms
are multi-layered colonies of bacteria that are typically more resistant to the host immune response and routine
antibiotic therapy. Biofilm has negatively impacted in health and medicine. Thus, this study was aimed to
assess the biocompatibility of locally produced HA coated with gentamicin on osteoblasts and to determine the
efficacy of gentamicin-coated HA on Staphylococcus aureus biofilm. For in vitro, S. aureus strain and normal
human osteoblast cell line were used. MTT assays were performed to determine the effects of various doses of
gentamicin-coated HA (from 0.02 mg/ml to 10 mg/ml) on biofilm viability and also used to determine IC50
value of gentamicin-coated HA on osteoblasts to assess the cytotoxicity level of this coated HA. For in vivo, the
tissue immediately surrounding the implanted catheter was determined by using H&E staining post-implantation.
Gentamicin-coated HA is biocompatible with bone tissue (osteoblasts) in vitro. It showed no detectable IC50 and
the percentage of osteoblasts viability was high (75%) via MTT assay. It was also able to reduce the viability of
S. aureus biofilm. After seven days of post-implantation, results also showed no post-implantation inflammatory
reaction in rats. In conclusion, gentamicin-coated HA proved to be biocompatible with human osteoblasts and
effective in reducing the number of bacteria both in vitro and in vivo.
Keywords: Hydroxyapatite, staphylococcus aureus biofilm, MTT assays, gentamicin
A Rapid Dipstick Immunoassay based on Recombinant SAG1 for Detection of
Immunoglobulin G (Igg) and M (Igm) Antibodies in Human Toxoplasmosis
Prof. Dr. Hj. Wan Omar Abdullah
Ngah Zasmy Unyah, Hairul Bazli Hairuddin, Malina Osman, Rukman Awang Hamat and Khairul Anuar
Faculty of Medicine and Health Sciences, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8947 2365; wanomar@medic.upm.edu.my
A dipstick immunoassay (DIA) has been successfully developed to detect specific immunoglobulin G
(IgG) or IgM antibodies to human toxoplasmosis. These assays employ nitrocellulose membrane (NCP) as a
test matrix, peroxidase conjugated to sheep anti-human IgG and rabbit anti-human IgM as detecting antibodies
and a Toxoplasma gondii recombinant SAG1 as the detective antigen. The assays are rapid (the whole test can
be completed within 15 min), simple, and cheap, and they do not require any equipment. They are sensitive and
specific for the detection of anti-Toxoplasma IgG or IgM antibodies and generally agree closely with the results
from the enzyme-linked immunosorbent assay. The assays are especially suitable for field applications.
Keywords: Dipstick, immunoassay, recombinant SAG1
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