BUKU ABSTRAK - Universiti Putra Malaysia

A Miracle of Mushroom: A Booster for Healthy Gut
Assoc. Prof. Dr. Shuhaimi Mustafa
Aida Firdaus Muhammad Nurul Azmi, Mohd. Hamim Hamdan Mustafa, Mohd. Yazid Abd. Manap
Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8941 6541; shuhaimi@biotech.upm.edu.my
Mushroom is a kind of fungi with a soft and fleshy body. More than 2000 species out of 12 000 species were
claimed to be edible. The ancient Chinese believed that, the consumption of mushroom (like shiitake) could help
in maintaining good health. Ganoderma lucidum is one of the famous mushrooms used in traditional Chinese
medicine. The therapeutic effects of these mushrooms are due to its polysaccharides content. Many researchers
have been conducted in other to prove the significant medicinal effect – antioxidant, antitumor, anticancer – of
mushroom scientifically. These include the discoveries of prebiotic properties from the crude polysaccharides of
G. lucidum (GLCP). GLCP were obtained using hot water extraction method. Prebiotic activity of GLCP was
tested against probiotic bacteria namely: Bifidobacterium longum BB536 in 10 mL basal Trypticase Phytone
Yeast (abbreviated as bTPY) medium (without glucose) supplemented with various concentrations of GLCP
(abbreviated as bTPYglcp) (0.5%, 1.0%, 1.5% and 2.0%). bTPY medium supplemented with glucose (abbreviated
as bTPYglu) and inulin (abbreviated bTPYinu) were used as comparison. Viable cell counts of the bacteria and
the pH of the medium were determined during anaerobic incubation at 0h, 12hours, 24hours and 48hours at 37°C.
In the presence of carbohydrate source, cultures showed various degree of growth increment. With regard to
the growth supporting property: B. longum BB536 showed greater growth in medium supplemented with 0.5%
GLCP (8.63 log cfu/mL) as compared to basal medium (7.31 log cfu/mL) at 24hours of incubation time. These
gave about 30% probiotic growth increment (in bTPYglcp) and only 10% increment in bTPY. The pH was noted
to drop from 6.8 to 4.5 in bTPYglcp and pH 6.8 to 5.8 in bTPY after 24hours of incubation. These showed that
probiotic bacteria was favoured to survive in medium supplemented with GLCP three time greater as compared
to the basal medium.
Keywords: Mushroom, prebiotic, gut health
Halal Encapsulation for Bifidobacteria (PI 2010001376)
Assoc. Prof. Dr. Shuhaimi Mustafa
Khalilah Abdul Khalil, Mohd. Yazid Abd. Manap, Arbakariya Ariff and Rosfarizan Mohamad
Institute of Products Halal Research, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8941 6541; shuhaimi@biotech.upm.edu.my
This invention describes encapsulation process for bifidobacteria delivery in a mammal (preferably human
or animal), where the bead includes Bifidobacterium pseudocatenulatum G4 and encapsulation matrix. The
encapsulation matrix includes gelatin, a cross linker and sodium alginate. The encapsulation matrix showed
good entrapping properties to the bacterium tested. The comparison of Bifidobacterium pseudocatenulatum G4
encapsulation yields (%) between bovine gelatin-containing matrix and porcine gelatin-containing matrix showed
no significant different. Therefore, the halal encapsulation matrix/process developed in this study offers promising
vehicle for the delivery of bifidobacteria and other active micromolecules into the targeted site which is the colon.
Keywords: Halal, bifidobacteria, delivery system
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