BUKU ABSTRAK - Universiti Putra Malaysia

Science, Technology & Engineering
Molecular Evaluation and Taxonomic Implications of Selected Malaysian Isolates
of Trichoderma harzianum
Mr. Shafiquzzaman Siddiquee
Umi Kalsom Yusuf and Tan Soon Guan
Faculty of Science, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 6620; shafiqpab@yahoo.com
Trichoderma species are commercially applied as biocontrol agents against some plant pathogenic fungi
due to their production of antifungal metabolites, competition for nutrients and space, and mycoparasitism.
Antagonistic assessment by dual culture technique showed that 18 out of 48 selected T. harzianum isolates
successfully inhibited the mycelial growth of Ganoderma boninense (isolate: PER71) at 47.86 to 72.06% with the
strongest inhibitor exhibited by strain FA30. Eight samples produced effective volatile antifungal compounds of
24.528 to 58.70 % over six days. When the 10 samples were assayed for the production of non-volatile antifungal
compounds, whereby showed the inhibitory effects of 18.35 to 40.16%. Strain FA30 was the best inhibitor
isolate not only by dual culture inhibition technique, but was also the best producer of volatile and non-volatile
inhibitor compounds. Identifications of Trichoderma species are currently misleading worldwide from micromorphological
descriptions which are tedious and prone to error. This study undertook a molecular approach
using isozyme, random amplified microsatellite (RAMS) and gene sequencing of internal transcribed spacer-1
(ITS 1) region of the rDNA. Molecular studies by isozyme and RAMS analysis did not give any confident level
separation at the species level. Gene sequencing of the ITS 1 region of the nucleotide sequences of samples was
multiple aligned and compared against the ex-type strains sequences from the NCBI and TrichoBLAST Genbank
database. Results showed that 25 out of the 26 putatively identified T. harzianum were inagreement with the
genomic of T. harzianum ex-type strain, except single strain belonged to T. virens. The 9 putative T. aureoviride
were misidentifications whereas 7 were T. harzianum and 2 were T. virens based on the GenBank database. The
ITS 1 region study showed that the gene sequences of Trichoderma samples were the most accurate technique for
identification at the species level.
Keywords: Genus Trichoderma, RAMS, isozyme analysis, internal transcribed spacer
ROTAZIONE: New Conceptual Occasional Table
Mr. Shahrul Azman Shahbudin
Mohd. Yusof Abdul Aziz, Mohd. Shahrizal Dolah, Bakri Bakar and Saiful Hasley Ramli
Faculty of Design and Architecture, University Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
+603-8946 4054; shazman67@yahoo.com
Occasional tables and the ubiquitous “coffee table” are quite essential partners to the sofa, not only for
displaying stylish art book and table lamps, but also for resting the more mundane mug of coffee and remote
control for the television. This research focuses on the value of furniture and the problem that occurs in current
living. It also focuses on using reused material in furniture making. The innovation targets users who are
executives who need to make the best use of space, an impact of new technological and social change.
Keywords: Occasional tables, coffee table, furniture, reuse material
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