27.03.2013 Views

BUKU ABSTRAK - Universiti Putra Malaysia

BUKU ABSTRAK - Universiti Putra Malaysia

BUKU ABSTRAK - Universiti Putra Malaysia

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Forestry & Environment<br />

Fungal Diversity in Wounded Stems of Aquilaria malaccensis<br />

Dr. Rozi Mohamed<br />

Jong Phai Lee and Mohammed Syazwan Zali<br />

Faculty of Forestry, University <strong>Putra</strong> <strong>Malaysia</strong>,<br />

43400 UPM Serdang, Selangor, <strong>Malaysia</strong>.<br />

+603-8946 7183; rozimohd@putra.upm.edu.my<br />

Aquilaria malaccensis is a tropical tree which produces agarwood in its trunk often after being wounded<br />

and attacked by pathogens or insects. Fungi are generally viewed as the main microbial component responsible<br />

for agarwood formation. In this study, isolation of fungi from agarwood in damaged trees was carried out.<br />

Culture morphology and microscopic characteristics plus PCR amplification of the internal transcribed spacer<br />

(ITS) region from the fungal isolates as well as wood samples,were used to identify the fungal community<br />

composition of wounded A. malaccensis trees from a natural forest in West <strong>Malaysia</strong>. Conventional culture<br />

methodology revealed Cunninghamella, Curvularia, Fusarium and Trichoderma species as members of the<br />

agarwood community. Analysis of genomic DNA confirmed the identifications. When wood samples were used<br />

directly in PCR, an additional Lasiodiplodia species was identified. Neighbor-joining trees were constructed to<br />

examine the relationships between the isolates sequence data and reference sequences in GenBank. Five distinct<br />

clades resulted, supported with high bootstrap values, indicating the presence of five distinct taxa. The wounded<br />

trunks of A. malaccensis in the natural environment harbor multiple fungal taxa that exist in a complex system as<br />

a whole or in succession leading to agarwood production in the tree trunk.<br />

Keywords: Agarwood, aquilaria malaccensis, fungi, phylogeny, production, wound<br />

Effect of Logging Operations on Soil Carbon Storage of a Tropical Peat Swamp<br />

Forest in Sarawak<br />

Dr. Seca Gandaseca<br />

Anton Eko Satrio, Osumanu Haruna Ahmed and Nik Muhamad Ab. Majid<br />

Faculty of Agriculture and Food Sciences, University <strong>Putra</strong> <strong>Malaysia</strong>,<br />

43400 UPM Serdang, Selangor, <strong>Malaysia</strong>.<br />

+603-8685 5473; secags@yahoo.com<br />

It is important to investigate the severity of degradation of peat swamp forest caused by logging operation<br />

in terms of its function as carbon storage. The peat soil was sampled randomly using a peat auger to a depth of<br />

15 cm. Soil sampling ware conducted before and after logging (one year later). Soil sampling was conducted<br />

on two different plots under different skidding systems to compare the soil carbon storage between kuda-kuda<br />

and excavator systems. Unpaired t-test was used to compare soil carbon storage under the two systems. The<br />

humic acid extraction was done and soil carbon storage values obtained by calculation. The calculation of soil<br />

carbon storage was done by the bulk density method. There was more unstable carbon on logged peat swamp<br />

forest. Afterwards, that high unstable carbon formed on logged peat swamp forest was converted into humic acid<br />

and stable carbon through humification processes. However, the similarity of stable carbon of the logged peat<br />

swamp forest with stable carbon of undisturbed peat swamp forest indicated an ineffectiveness humification of<br />

logged peat swamp forest. Under kuda-kuda skidding system, unstable carbon seemed to be preserved better and<br />

this was because of the better carbon storage. After the peat swamp forest was logged, humification processes<br />

were strongly maintained, but it become slow and ineffective, hence unstable carbon decomposed more instead<br />

of it being preserved as stable carbon. The skidding systems altered the unstable carbon. The unstable carbon<br />

decomposed more under excavator skidding system, but inversely under kuda-kuda skidding system.<br />

Keywords: Peat swamp forest, logging operation, skidding system, humification, soil carbon storage<br />

96

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!