sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

SDÜ ORMAN FAKÜLTESİ DERGİSİ
2.2. Analyzes of amplification products
Amplification products were separated by electrophoresis (90 V for 120 min) in
1.5 % agarose gels in TAE buffer and length of the products were determined by
using 100 bp DNA Ladder. The presence or absence of amplification products was
scored to analyze the variation among the populations.
3. RESULTS
The amplifications yielded a total of 18 markers ranging from 300 to 1200 bp in
size. The amplification profiles were very similar for most of the
Gymnosporangium telial horns; the M13 primer seemed not detect any significant
level of variation within 50 samples.
Figure 2: Amplification products of G. fuscum isolates from different sites.
4. DISCUSSION
The very low level of variation detected by M13 amplifications was surprising.
Within its 2- year life cycle G. fuscum produces relatively limited number of
genetically identical spores. Each aecidium on pear is initiated by a single
basidiospore and therefore could be expected to be (at least in practise) genetically
different from other aecidia. As the fungus lacks an uredinial stage on pear the
number of infections on pear caused by single genets is not multiplied. As a result,
genetic variation among aecidiospores infecting junipers should be high. As new
infections on juniper occur annually, one could expect to find a high level of
genetic variation among G. fuscum isolates on that host.
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