sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

SDU Faculty of Forestry Journal
Serial: A, Number: Special Issue, Year: 2009, ISSN: 1302-7085, Page: 257
NEW ADVANCES IN THE STUDY OF THE TAXONOMY OF THE
EUROPEAN RACE OF GREMMENIELLA ABIETINA
Leticia BOTELLA 1 , Julio Javier DIEZ 1 , Jarkko HANTULA 2*
1 Laboratorio de Entomología y Patología Forestal, Departamento de Producción Vegetal
Recursos Forestales, E.T.S.I.I.A.A., Universidad de Valladolid, “Campus La Yutera”, Avda. Madrid,
44, 34004, Palencia, Spain.
2 Finnish Forest Research Institute (METLA), Unit of Vantaa. Joikiniemenkuja, 1, PL 18, FI-
0130. Finland.
* jarkko.hantula@metla.fi
In order to investigate the taxonomy of the European race of Gremmeniella
abietina var. abietina ten Spanish isolates were randomly chosen among 91 to be
analyzed genetically and compared with 7 Swiss isolates biotype Alpine, 10
Finnish isolates biotype A and 10 Finnish isolates biotype B randomly chosen in
the same way. RAMS markers CCA, CGA and GAAA1000 supported by sequence
analysis of the locus GAAA1000 were used in this report to study the genetic
variability of Spanish and Swiss isolates in respect of the rest of European biotypes
and, thus, to establish what biotype they belong to. Philogenic relationships among
A, B, Alpine and Spanish sequences analyzed based on the neighbour-joining
method showed that Alpine type, that is, Swiss sequences, is more closely related
to B type, and Spanish isolates appear clearly separated from the rest of the
biotypes.
Keywords: Brunchorstia pinea – European race – Pinus halepensis – RAMS
– taxonomy.
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