sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

SDÜ Faculty of Forestry Journal
influenced the ‘treatment’ effect (interaction time*treatment: F = 3.94, p < 0.01).
LSD test showed the inoculation with endophytes 08-10 and 08-13 to produce a
significantly lower symptom severity caused by the second isolate (p2) of D. pinea
after 2- and 4-weeks of incubation but only when compared with the control
seedlings non-wounded in the lower part (Fig. 3). However, those fungi did not
cause reduced symptom severity when compared with those caused by Diplodia on
the controls wounded in the lower part and inoculated with PDA instead of
endophyte mycelia (Controls PDA). For the most aggressive isolate of D. pinea
(the p1 isolate), no endophyte was able to reduce significantly the symptom
severity caused by Diplodia (Fig. 3). Re-isolation percentages of each fungus can
be observed in Table 2.
Figure 3: Mean necrosis length caused by the D. pinea for each endophyte treatment
after 2 (figure a) and 4 weeks (figure b) of incubation. Averages with the same letter are
not significantly different according to Fisher´s least significant difference (LSD) test at
a significant level of 0.05. Vertical bars indicate Standard Error. ‘Control’ was not
wounded 5 cm below the apex; and ‘Agar’ was a control wounded and inoculated with
agar 5 cm below the shoot apex.
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