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sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

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Eggs hatching (%)<br />

100<br />

50<br />

0<br />

SDÜ Faculty <strong>of</strong> Forestry Journal<br />

0 2 4 6 8 10 12 14 16 18 20 22<br />

198<br />

days<br />

Control-25<br />

Mycotal-25<br />

Figure 4: Percentage <strong>of</strong> Cryptococcus fagisuga’s eggs hatching after treatment with<br />

Lecanicillium muscarium (Mycotal®) over a 21-day period.<br />

4. CONCLUSION<br />

The scale insect Cryptococcus fagisuga, exposed to Lecanicillium muscarium<br />

(Mycotal®) and Beauveria bassiana under laboratory conditions, reduced the noncrawling<br />

nymphal stage population by 50%. This percentage could have been<br />

higher, but the experiment on bark disks cannot last longer than 10 to 12 days;<br />

because <strong>of</strong> the high rate <strong>of</strong> humidity, fungal hyphae invade the disks making<br />

further observations impossible. The fall population treated with entomogenous<br />

fungi had a mortality rate reaching 50% in 10 days. Scale insects collected in<br />

winter show a higher rate <strong>of</strong> mortality after biological treatment; the difference<br />

with fall is probably the mortality caused by the very cold temperatures during the<br />

winter. Finally, if eggs are not directly invaded by fungal hyphae, the young<br />

crawlers are rapidly colonized by the fungi soon after hatching (data not shown).<br />

Field trials with these biological control agents will be the next step <strong>of</strong> this study.<br />

5. LITERATURE CITED<br />

Castlebury, L.A., Rossman A.Y., Hyten A.S., 2006. Phylogenetic relationships <strong>of</strong><br />

Neonectria/Cylindrocarpon on Fagus in North America. Can. J. Bot. 84, 1417-1433.<br />

Crosby, D., Bjorkbom J.C., 1958. Timely salvage can reduce losses from beech scale Nectria attack.<br />

U.S. Forest Serv., Northeastern Forest Experiment Station, Forest Research Note 82, 4p.<br />

Ehrlich, J., 1934. The beech bark disease. A Nectria disease <strong>of</strong> Fagus, following Cryptoccus fagi<br />

(Baer.). Can. J. Res. 10, 593-692.<br />

Evans, C.A., Lucas J.A., Twery M.J. (Eds.), 2005. Beech Bark Disease: Proceedings <strong>of</strong> the Beech<br />

Bark Disease Symposium. General Technical Report NE-331. Newtown Square PA, US<br />

Department <strong>of</strong> Agriculture Forest Service, Northern Research Station, 149 p.

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