sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
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SDÜ Faculty <strong>of</strong> Forestry Journal<br />
As the results are preliminary we can not exclude the possibility <strong>of</strong> non-optimal<br />
conditions in the PCR reactions. However, the amplicon pr<strong>of</strong>iles looked normal<br />
without signs <strong>of</strong> e.g. abnormally long fragments. Therefore other explanations are<br />
more likely.<br />
Arbitrarily primed PCR using M 13 core sequence as a primer detects variation<br />
in several <strong>of</strong> species <strong>of</strong> fungi growing on forest trees including such as;<br />
basidiomycetes Heterobasidion annosum s.l. (Fr) Bref. (Hantula et al., 1996;<br />
Stenlid et al., 1993)and Fomitopsis pinicola (Schwarts: Fr) Karst. (Högberg et al.,<br />
1995), ascomycetes Ophiostoma ulmi (Buism), Ceratocystis fimbriata f.sp. platani<br />
(Santini et al., 2000), and Nectria fuckeliana C. Booth (Vasiliauskas and Stenlid,<br />
1997) and Sphaeropsis sapinea Dyko& Sutton (Xiaoqin et al., 2007) among fungi<br />
imperfecti. Among biotrophs the primer has been used successfully for powdery<br />
mildews (ascomycete) but to our knowledge not for smuts and rusts<br />
(basidiomycetes). It is possible that annealing sites for the M13 primer within G.<br />
fuscum genome are few. Another reason for the low variation could be that the<br />
distances between the sampled stands were short.<br />
Table 1. Presence and absence vector <strong>of</strong> amplification products.<br />
Isolates 330bp 360bp 380bp 390bp 400bp 450bp 540bp 550bp 590bp 650bp 660bp 680bp 710bp 810bp 870bp 910bp 950bp 1200bp<br />
S1.1 1 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0<br />
S1.2 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0<br />
S1.3 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0<br />
St2.1 1 0 1 0 0 0 1 0 0 1 0 0 0 0 0 1 1 1<br />
St2.2 1 1 0 0 0 0 1 1 0 1 0 0 0 1 0 0 0 0<br />
St2.3 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0<br />
St2.6 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0<br />
B3.1 0 1 0 0 0 0 1 1 0 1 1 1 0 0 1 0 0 1<br />
B3.2 0 1 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 1<br />
B3.3 0 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0<br />
B3.4 0 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0<br />
B4.4 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 1 1<br />
Be4.5 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 1 1<br />
Be4.6 1 0 0 0 0 0 1 1 0 1 0 0 0 0 0 1 0 0<br />
Be5.8 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0<br />
Be5.9 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0<br />
Be5.10 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0<br />
5. ACKNOWLEDGEMENTS<br />
This work was supported by State Planning Organisation <strong>of</strong> Turkish Republic<br />
(DPT–2003 K 1211020-7).<br />
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