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sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

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SDÜ ORMAN FAKÜLTESİ DERGİSİ<br />

samples. Samples were mixed for each plot, sieved to pass 2 mm after air drying,<br />

and physical and chemical properties were determined in laboratory.<br />

2.3. Needle samples<br />

Prior to sampling, every tree was labelled and their health condition was<br />

recorded. Five healthy and 5 unhealthy trees were chosen for foliar sampling in<br />

each plot (Figure 1). 40 fascicles were taken from each tree with a shotgun.<br />

Samples were dried at 70°C for 24 hours. The dry weights were measured and the<br />

needles ground, then chemical analysis was performed for nutrients.<br />

2.4. Plant water potential<br />

We assumed that water potential <strong>of</strong> the trees may have played a role in<br />

development <strong>of</strong> this fungal disease. Five trees were chosen in each plot for water<br />

potential measurements. From each tree, representative needle samples were taken<br />

from the bottom 1/3 <strong>of</strong> the canopy, and needle water potentials were measured with<br />

a pressure bomb. However, the data was not conclusive, so the initial results were<br />

not included here.<br />

3. RESULTS<br />

3.1. Fungal survey<br />

Figure 1. Healthy (a) and unhealthy (b) pine needles.<br />

Since start <strong>of</strong> the fungal disease in 2004, the rate <strong>of</strong> the disease has decreased<br />

over time. In 2006, the ratio <strong>of</strong> samples with Diplodia was 20 %, while in 2008 the<br />

ratio fell down to 5.5 %. As <strong>of</strong> today, the stand looks quite healthy.<br />

3.2. Soil samples<br />

On average, soil texture was sandy clay loam (Table 1). Soil reaction was relatively<br />

neutral. Organic matter and nutrient concentrations were moderate. However, soil<br />

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