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sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

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SDÜ ORMAN FAKÜLTESİ DERGİSİ<br />

June 30. On each date, the number <strong>of</strong> leaves per twig was assessed, and twigs were<br />

sprayed until run<strong>of</strong>f with each treatment (up to 5 mL per twig, but depending on<br />

the number <strong>of</strong> leaves and leaf sizes). A plastic board was held behind each twig<br />

while spraying to prevent overspray onto other twigs. There were nine chemical<br />

treatments and a water control (Table 1).<br />

Table 1: Treatments applied to 2 m tall maple trees at the Guelph Turfgrass Institute,<br />

Guelph, Ontario, Canada, in Spring 2008 for control <strong>of</strong> tar spot.<br />

Treatment Common Name Product/ L<br />

Water control water 0 ml<br />

Banner MAXX propiconazole (15.6%) 0.245 mL<br />

Compass 50WG trifloxystrobin (0.16%) 0.175 g<br />

Daconil Ultrex chlorothalonil (82.5%) 1.5 g<br />

Dithane DG mancozeb (75%) 3 g<br />

Eagle WP (Nova) myclobutanil (40%) 0.34 g<br />

Heritage WG azoxystrobin (50%) 0.3 g<br />

Rovral Green GT iprodione (25%) 10 mL<br />

Senator WP thiophanate-methyl (70%) 1 g<br />

Sulfur sulfur (92%) 10 g<br />

The number <strong>of</strong> spots per leaf was assessed weekly from the start <strong>of</strong> the trial<br />

until the end <strong>of</strong> June, and then assessed monthly until the end <strong>of</strong> September. The<br />

morphology and phenological state <strong>of</strong> the maple leaves was also recorded during<br />

each assessment.<br />

3. RESULTS AND DISCUSSION<br />

As observed in previous years (Hsiang & Tian 2008), the first symptoms <strong>of</strong> tar<br />

spot on Norway maple in Southern Ontario appeared in late June as small, round,<br />

light green, chlorotic spots, 2 mm across. Spots enlarged to 15 mm by mid-August,<br />

and developed small black tar-like raised structures on the adaxial surface with a<br />

yellow margin. Conidia, which are considered non-infective and possibly<br />

spermatizing, appeared as a shiny layer on the black stroma at this time. By early<br />

September, the individual spots merged into a circular black spot up to 2 cm across.<br />

Overwintered Norway maple leaves collected in March 2007 and 2008, had<br />

stroma, paraphyses and asci (56-80 µm × 8.5-10.6 µm), but no ascospores were<br />

visible. By the middle <strong>of</strong> April, the asci were still undifferentiated, but were found<br />

to contain globular vacuoles or bodies. The asci became swollen as spores<br />

developed, and filiform ascospores were first observed in early May, averaging 55<br />

× 2.0 µm. By late May, after soaking in water, slits in the hysterothecia (modified<br />

191

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