sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
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SDÜ Faculty <strong>of</strong> Forestry Journal<br />
understand the penetration capacity <strong>of</strong> the fungus to the healthy tissue. Also in the<br />
present study more realistic results <strong>of</strong> the virulence <strong>of</strong> the isolates could have been<br />
obtained by spraying the seedlings with conidial suspensions.<br />
Virulence <strong>of</strong> S. sapinea s.l. isolates on pine varies depending on several factors,<br />
such as ecological and climatic conditions, season, species <strong>of</strong> the fungus and where<br />
the experiment takes place (Swart and Wingfield, 1991; Blodgett and Stanosz,<br />
1997). It is also known that S. sapinea s.l. is able to live without typical symptoms<br />
in its latent phase (Stanosz et al., 1997; Flowers et al., 2001; 2003; Stanosz et al.,<br />
2005; Maresi et al., 2007). In that sense, it is important to have knowledge about<br />
the latent infections in nurseries and forest stands. D. scrobiculata was not found in<br />
the area where the isolates used in the present experiment were collected. As the<br />
isolates <strong>of</strong> D. pinea were directly obtained from pycnidia located on diseased<br />
shoots, instead <strong>of</strong> isolating from host tissues, only the frequently fruiting species<br />
could be found. Since the asymptomatic persistence <strong>of</strong> the fungal species in the<br />
host tissues is important for their wide distribution, a study focusing in latent<br />
infections is needed to shed light on the Diplodia-species composition on the local<br />
tree species and their infection potential.<br />
5-ACKNOWLEDGEMENTS<br />
This research was founded by Süleyman Demirel University Scientific Projects<br />
Unit (1325-06) and Republic <strong>of</strong> Turkey Prime Ministry State Planning<br />
Organisation (DPT 2003- K- 121020 /7). We are also very grateful to Glen Stanosz<br />
and Denise Smith for their help in molecular identification <strong>of</strong> isolates <strong>of</strong> S. sapinea<br />
sensu lato, Department <strong>of</strong> Plant Pathology, University <strong>of</strong> Wisconsin-Madison.<br />
REFERENCES<br />
Adams, G. C., Wu, N. –T., Eisenberg, B. E., 2002. Virulence and double-stranded RNA in<br />
Sphaeropsis sapinea. Forest Pathology 32 (2002) 309–329.<br />
Blodgett, J.T., Stanosz, G.R., 1997. Sphaeropsis sapinea morphotypes differ in aggressiveness, but<br />
both infect nonwounded red or jack pines. Plant Disease 81, 143-147.<br />
Blodgett, J.T., Bonello, P., 2003. The aggressiveness <strong>of</strong> Sphaeropsis sapinea on Austrian pine varies<br />
with isolate group and site <strong>of</strong> infection. Forest Pathology 33, 15-19.<br />
Burguess, T., Wingfield, M. J., 2001. Simple sequence repeat markers distinguish among<br />
morphotypes <strong>of</strong> Sphaeropsis sapinea. Applied and Environmental Microbiology 67, 354-<br />
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Brookhouser, L. W. Peterson, G. 1971. Infection <strong>of</strong> Austrian, Scots, and panderosa pines by Diplodia<br />
pinea. Phytopathology 61, 409–414.<br />
de Wet, J., Burgess, T., Slippers, B., Preisig, O., Wingfield, B.D., Winfield, M.J., 2000.<br />
Characterization <strong>of</strong> Sphaeropsis sapinea isolates from South Africa, Mexico and<br />
Indonesia. Plant disease 84, 151-156.<br />
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