sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

SDÜ ORMAN FAKÜLTESİ DERGİSİ
Table 1. Linear extension of D. pinea in the shoots of five tree species inoculated with
different isolates (mm).
Tree
species
53
Isolates
T008 T029 P047 T081 P097 Control Averages
A.n.bor. 6.0 B ab 8.0 B a 10.0 BC a 10.0ABC a 8.0 B a 0.0 Ab 8.4AB
C. libani 12.0 AB b 34.0 A a 14.0 B b 20.0AB ab 12.0 B b 0.0 Ac 18.4 BC
J. excel. 2.0 B a 2.0 B a 2.0 C a 2.0 C a 0.0 C a 0.0 Aa 1.6 A
P. brutia 20.0 A ab 38.0 A a 20.0 B ab 24.0 A b 18.0AB ab 0.0 Ac 24.0 BC
P. nigra 22.0 A abc 22.0ABabc 54.0 A a 6.0 BC bc 34.0 A ab 0.0 Ac 27.6 C
P.syl. 12.0AB ab 8.0 B bc 28.0 AB a 24.0 A ab 10.0 B abc 0.0 Ac 16.4 ABC
Means in the same column followed by the same uppercase letter and means in the same row
shown by the same lowercase letter were not significantly different from each other according to
Duncan’s Multiple range test (P=0.05)
4- DISCUSSION
Inoculations with the five D. pinea isolates resulted in necrotic needles and dead
shoot tips on the tested coniferous tree species, showing high virulence on C. libani
and P. nigra and low on A. nordmanniana ssp. bornmülleriana and J. excelsa.
Even though most of the coniferous tree species have been tested for their
sensitivity to S. sapinea sensu lato (s.l.) (Chou, 1976; Brookhouser, 1971; Blodgett
and Stanosz, 1997; Flowers, 2001; Blodgett and Bonello, 2003; Luchi et al., 2007;
Munoz et al., 2007) this is the first study mentioning the high susceptibility of C.
libani to D. pinea isolates.
The time of the inoculation significantly affects the relative aggressiveness of
the fungus. (Literatür). The results of the experiment indicate that D. pinea were
pathogenic on tested conifer tree species, especially on C. libani and Pinus spp.
However, inoculations with most of the fungal isolates resulted in little or no
measurable symptoms on shoots of J. excelsa and A. nordmanniana in October and
November when the experiment was carried out. To confirm these results it may be
necessary to repeat the experiment during spring when the conditions are much
more favorable for the natural spread of the fungus.
Blodgett and Stanosz (1997) indicated that the wound inoculation technique
provides a reproducible method for comparing the aggressiveness of the isolates
belonging to different species of the fungus but, they did not found this method
reliable for comparing the aggressiveness of the isolates within the species. In the
present experiment, the same technique was used to find out the susceptibility of
the host species to the disease, and similarly, there were no significant differences
between the five isolates on any of the tree species. Blodgett and Stanosz (1997)
conducted the inoculation experiments also with conidial suspensions to be able to