sdu faculty of forestry journal special edition 2009 - Orman Fakültesi

SDÜ ORMAN FAKÜLTESİ DERGİSİ
Fig. 2. Distribution of Lecanosticta acicola in Europe. Years mean first report, in
parentheses are years of findings, if differ from year of publishing.
2. MATERIAL AND METHODS
Records from the Czech Republic are based on monitoring carried out in 2000 –
2008. Pine and also Spruce and Douglas fir needle samples were examined, mainly
from regions of Southern and Central Moravia, Silesia, Eastern and Central
Bohemia.
The presence of the pathogen was always investigated according to
characteristic symptoms such as red bands, dying tips of needles or the occurrence
of subepidermal sporocarps, acervuli. Exact identification was proved on the basis
of microscopic analyses of conidia.
Isolation of culture was made on 3% MEA containing malt extract 30 g/l,
pepton 5 g/l, agar 15 g/l, without addition of any antibiotics. Pieces of needles with
acervuli 3 - 5 mm long were on surface sterilized by sodium hypochlorite 7%,
subsequently by ethanol 96% and washed by sterilized water and put on malt
extract agar. After 3 weeks of incubation, when new conidia occurred on fruiting
bodies, conidia were inoculated into new medium.
Herbarium specimens are deposited at Herbarium of Faculty of Forestry and
Wood Technology (BRNL).
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