sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
sdu faculty of forestry journal special edition 2009 - Orman Fakültesi
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SDÜ ORMAN FAKÜLTESİ DERGİSİ<br />
In the case <strong>of</strong> red pine seedlings, two-way ANOVA <strong>of</strong> the symptom severity<br />
showed a significant effect <strong>of</strong> the treatment (F = 15.89, p < 0.01), the trial (F =<br />
16.29, p < 0.01), but not <strong>of</strong> their interaction (F = 0.34, p = 0.88). Therefore,<br />
multiple comparison was performed separately for each trial (Figure 2). As in the<br />
case <strong>of</strong> jack pine, isolates ranked in the same order in relation to their<br />
aggressiveness, although in general terms, symptom severity was lower in red pine<br />
than in jack pine. In red pine, it was also observed that the presence <strong>of</strong> either isolate<br />
<strong>of</strong> D. scrobiculata in the seedling reduced the symptom severity caused by either<br />
isolate <strong>of</strong> D. pinea (except in trial 1 for P2 isolate), although in red pine, the<br />
differences between treatments were not so evident (Figure 2). Molecular analyses<br />
indicated that symptom severity was caused mainly by D. pinea. D. scrobiculata<br />
was only detected in the proximities <strong>of</strong> the inoculation site.<br />
Figure 2: Mean symptom severity (distance below the inoculation each trial on red<br />
pine seedlings. Averages with the same letter are not significantly different according to<br />
Fisher´s least significant difference (LSD) test at a significant level <strong>of</strong> 0.05. Vertical<br />
bars indicate Standard Error.<br />
3.2. Interaction between D. pinea and several endophytes experiment<br />
Jack pine seedlings inoculated with D. pinea isolates also produced symptoms<br />
similar to those reported above. No symptoms developed on nonwounded controls.<br />
After 2 weeks, the two-factor analyses <strong>of</strong> variance <strong>of</strong> the ‘necrosis length’ (distance<br />
below the inoculation site at which necrotic needles were present) indicated a<br />
significant effect <strong>of</strong> the ‘treatment’ (F = 4.24, p < 0.01), the trial (F = 6.53, p =<br />
0.012), but not <strong>of</strong> the interaction (F = 1.41, p = 0.18). The two-factor ANOVA <strong>of</strong><br />
the ‘necrosis length’ caused by Diplodia isolates after 4 weeks showed that only<br />
the ‘treatment’ effect was significant (F = 2.91, p < 0.01). Only the ‘Time’ effect<br />
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