Principles of Fluorescence Spectroscopy

PRINCIPLES OF FLUORESCENCE SPECTROSCOPY 149
Figure 4.63. Tryptophan intensity decays of FKBP59-1 excited by
synchrotron radiation at 295 nm and observed at 310 and 380 nm.
Revised from [219].
near 6 ns in the presence of lipid. It is easier to visualize the
effects of lipid from the distributions than from a table of
parameter values.
Lifetime distributions and the MEM are also useful in
visualizing the effects of observation wavelength on intensity
decays. 219 The immunophilin FKBP59-1 contains two
tryptophan residues, one buried and one exposed to the solvent
(Chapter 16 and 17). The intensity decays are visually
similar on the short (310 nm) and long (380 nm) sides of the
emission spectrum (Figure 4.63). The difference between
the decays are much more apparent in lifetime distributions
recovered from the maximum entropy analysis (Figure
4.64). An excellent monograph has recently been published
on TCSPC [220]. Readers are encouraged to see this book
for additional details on TCSPC.
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Figure 4.64. Lifetime distribution of the immunophilin FKBP59-1
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