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Principles of Fluorescence Spectroscopy

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928 INDEX<br />

Correlation time distributions, 386–387, 393<br />

DNA binding to HIV integrase, 404–406<br />

Correlation time imaging, 406–408<br />

Correlation times, 353–354, 367–371, 422<br />

Cortisol, 661–662<br />

Cos cells<br />

fluorescence-lifetime imaging microscopy, 745–746<br />

Cosyntropin, 590<br />

Coumarin, 57–58, 73<br />

Förster distances, 468<br />

Coumarin 108, 151–152, 222<br />

Coumarin-based potassium probe, 646–648<br />

Coumarin derivatives<br />

fluorescence-lifetime imaging microscopy, 748<br />

photoinduced electron transfer quenching, 338–340<br />

quenching, 284<br />

solvent relaxation, 249–250, 253<br />

Coum-Eu, 189–190<br />

Covalent adduct formation, intramolecular quenching, 314<br />

C-peptide, 813–814<br />

CRABPI (retinal binding protein), 560<br />

Crambin, 535<br />

Cramer's rule, 198<br />

Creatine kinase, 662–663<br />

Cresyl violet, 54<br />

Creutzfeldt-Jakob disease, 827<br />

Critical concentration, 466<br />

Cross-correlation in flow measurements, 831<br />

Cross-correlation method, 164–167, 177, 194<br />

Crown ethers, 17, 643–645, 647, 649<br />

C-Snafl 2, 127–128<br />

Cy3, metal-enhanced fluorescence, 848–851<br />

Cy3.5, 746–747<br />

Cy5.5, 657–658, 660–661<br />

Cyan fluorescent protein (CFP), 458, 497, 654<br />

Cyanine dyes, 74–75, 512–513<br />

immunoassays, 641<br />

potassium sensors, 635<br />

9-Cyanoanthracene, 185–186, 255, 279, 364, 744<br />

frequency-domain lifetime measurements, 170–171<br />

3-Cyanoperylene (PeCN), 129<br />

Cy5 (carboxymethylindocyanine-N-hydroxysuccinimidyl ester),<br />

780, 787, 824<br />

acceptor, 680<br />

Förster distances, 468<br />

metal-enhanced fluorescence, 848–851<br />

Cyclic AMP receptor protein (CRP), 291–292<br />

Cyclodextrins<br />

phosphorescence <strong>of</strong> bound probes, 317<br />

quenching, 286<br />

solvent effects, 227–228<br />

Cyclohexane, indole emission spectra in cyclohexane-ethanol<br />

mixture, 533–534<br />

Cysteine, 278, 651<br />

Cytotoxin II, 535<br />

D<br />

Dabcyl, 721–722<br />

Dabcyl acceptor, 724<br />

Danca, 243–244<br />

Dansylamide<br />

RET in three dimensions, 509–510<br />

Dansyl chloride, 16–17<br />

Dansyl groups, 16, 67–68<br />

anisotropy decay, 396–397<br />

creatine kinase immunoassay, 663<br />

energy transfer, 447–449, 451<br />

ethylenediamine, 495<br />

Förster distances, 447, 468<br />

solvent effects, 227–228<br />

DANZA, 662–663<br />

DAPI, 15, 16, 41, 75, 186–187, 359<br />

fluorescence-lifetime imaging microscopy, 750<br />

multiphoton excitation, 610–612<br />

Dapoxyl probes, 229<br />

Dapoxyl SEDA, 229–230<br />

DAS. See Decay-associated spectra (DAS)<br />

Data analysis, 129–130<br />

cross-fitting<br />

distance distribution, 481–482<br />

melittin lifetime distributions, 188–189<br />

frequency-domain lifetime measurements, 162–163<br />

literature references, 837<br />

time-domain lifetime measurement, 129–133<br />

maximum entropy method, 148–149<br />

DBO, 314<br />

DCM, 112<br />

DCVJ, 225<br />

d-d state, 685<br />

Dead time, 124<br />

time-to-amplitude converter (TAC), 115<br />

Debye-Sears modulator, 163<br />

Decay, 170–173. See also Intensity decay<br />

Decay-associated spectra (DAS), 581, 596–598<br />

barnase, 591<br />

frequency-domain data for calculation <strong>of</strong>, 269–270<br />

TRES and DAS comparison, 255<br />

two-state model, 263<br />

Decay time. See Lifetime (decay time)<br />

Deconvolution, 131<br />

Dehydroergosterol, 80<br />

Delay lines, 116<br />

Demodulation, 98<br />

Denaturation, 588<br />

Denatured pepsin, 806<br />

Denatured proteins, emission spectra for, 538<br />

Dendrimers and polymers<br />

literature references, 791<br />

DENS, 26. See also Dansyl groups<br />

Deoxycytidine quenching, 284<br />

Deoxynucleotide triphosphates (dNTPs), 729<br />

Depolarization, trivial causes, 365–366<br />

Depolarization factor multiplication, 436–437<br />

Depth-dependent quenching, 296–298<br />

Detection pr<strong>of</strong>ile, 802–803<br />

Detectors<br />

CCD, 49<br />

charged-coupled device, 766<br />

higher-frequency measurements, 176–177<br />

laser scanning microscopy, 749–750<br />

optical phase-sensitive, 743<br />

for single-molecule detection, 765–766<br />

single-photon-counting avalanche photodiode, 765<br />

time-correlated single-photon counting, 117–121<br />

color effects in, 119–121

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