Principles of Fluorescence Spectroscopy

942 INDEX
Naphthylamines and sulfonic acids
excited-state reactions, 610
quenchers of, 279
Naphthylamine sulfonic acids, 71. See also ANS; TNS
(2-(p-toluidinyl)naphthalene-6-sulfonic acid)
NATA (N-acetyl-L-tryptophanamide), 217
anisotropy decay of, 588
decay-associated spectra, 582
intensity decay, 120, 579–580, 581–582
lifetime reference, 100
quenching, 285, 341, 547, 548
rotational correlation times, 590
structure, 579
Natural fluorescence of proteins, 594–596
Natural lifetime, 10, 537
NATyrA (N-acetyl-L-tyrosinamide), 531–532
intensity decay of, 582–583
NBD derivatives
Förster distances, 468
lipids
quenching, 279, 299, 300
red-edge excitation shifts, 258–259
NBD (7-nitrobenz-2-oxa-1,3-diazole)
quenchers of, 279
NBS (nitrobenzenesulfenyl)
Förster distances, 468
Nd:YAG lasers, 108, 109–111
Near-field scanning optical microscopy (NFSOM),
763–764, 765, 778
literature references, 793
Near-infrared emitting lanthanides, 682, 683
Near-IR. See Light-emitting diodes
Near-IR probes, DNA sequencing, 710–711
Negative pre-exponential factor, 597
Nelder-Mead algorithm, 131
Neodymium, 682–683
Neodymium:YAG lasers, 110
Neuro-2a, 455
Neuronal receptor peptide (NRP), 551–552
Neutral density filters, 40
Neutral Red (NR), 223, 338, 339
N-Hydroxysuccinimide, 68
Nickel, 278, 279
Nicotinamide, 348
Nicotinamide adenine dinucleotide. See NADH
Nile Blue, 514–515
Nitric oxide, 278, 279
Nitrite, 348
Nitrobenz-2-oxa-1,3-diaxol-4-yl. See NBD
Nitrogen as flashlamp, 113
Nitromethane, 279
Nitrous oxide, 632–633
Nitroxide-labeled 5-doxylstearate, 299
Nitroxide-labeled phosphatidylcholines, 295, 296
Nitroxide quenching, 278, 279, 295
Nonlinear least-squares analysis. See
Least-squares analysis
Nonlinear least squares (NLLS), 124
Non-radiative decay, 842–843
Non-radiative decay rate, 222–223
Non-spherical fluorophores, 391
Notch filters, 39, 40
Nuclease, 548
Nucleic acids, 65
anisotropy decay, time-dependent, 402–406
DNA binding to HIV integrase, 404–406
DNA oligomer hydrodynamics, 403
intracellular DNA dynamics, 403–404
aptamers, 724–726
energy transfer, 459–461
labeling methods, 707–708
quenching, 284
Nucleotides, 65
quenching by, 341–342
O
Oblate ellipsoids of revolution, 422–423
Occupation numbers, 804
Octadecapentaenoic acid, 401–402
Octadecyl rhodamine B (ORB), 512–513
Octadecyl rhodamine (ODR), 519
Förster distances, 468
Octanol, 210, 215
Off-center illumination, 55
Olefins, 278, 279
Oligonucleotides
anisotropy decay, 416
energy transfer, 710
Oligosaccharides, distance distributions, 495–496
One-photon excitation, 171, 608–609
absorption spectra, 610
cross-sections for, 609
Optical bias, 166
Optical density, 55
Optical filters, 29, 766–768
bandpass, 39
colored, 38–39
combination, 40
for fluorescence microscopy, 41
interference, 38–39
neutral density, 40
signal purity and, 41–44
thin-film, 39–40
Optical properties
of metal colloids, 845–846
of tissues, 625
Optics
confocal, 761–762, 763
internal reflection, 760–761
single-molecule detection, 762–764
Oregon Green, 516, 639, 648
Orientation factor (k2 ), 448–450
energy transfer, 465, 489
on possible range of distances, 448–449, 452–453
Orientation imaging of R6G and GFP, 777–778
Orientation polarizability, 209, 211
Oriented fluorophores, transition moments,
353, 377–378
Oscillator strength, 59
Osmium metal–ligand complexes, 684, 685, 686
energy gap law, 688
Osmium MLCs, 88
Overlap integral, 445, 449–450
Oxazine dyes, 74
Oxonal, 73