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Principles of Fluorescence Spectroscopy

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PRINCIPLES OF FLUORESCENCE SPECTROSCOPY 619<br />

REFERENCES<br />

Figure 18.25. Three-dimensional cell imaging using confocal or multiphoton microscopy.<br />

Figure 18.26. Three-dimensional reconstruction <strong>of</strong> a live PC cell stained with acridine orange, which emits green (525 nm) when bound to DNA and<br />

red (650 nm) when present in acidic organelles. The perspective on the left is rotated 60° from the cells around the horizontal axis. The perspective<br />

on the right is rotated 90° around the vertical axis. Figure courtesy <strong>of</strong> Dr. Stefan Hall, Max-Planck Institute for Physical Chemistry, Göttingen,<br />

Germany.<br />

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