Principles of Fluorescence Spectroscopy

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874 APPENDIX I P CORRECTED EMISSION SPECTRA Table I.1. Properties <strong>of</strong> the Emission Intensity Standards* Emission Probe CAS Solvent Excitation range Tryptophan 54-12-6 Water 265 310–428 α-NPO 846-63-9 Methanol 315 364–486 TPB 1450-63-1 Cyciohexane 340 390–570 Coumarin 153 53518-18-6 Methanol 400 486–678 DCM 51325-91-8 Methanol 460 556–736 LDS 751 N/A Methanol 550 646–844 *CAS number (Chemical Abstract Service registry number) provides a unique identification for each fluorophore. The excitation wavelength and emission range is reported in nm. LDS-751 is a proprietary material <strong>of</strong> Exciton Inc. (Dayton, OH) and is similar to Styrl 8. Coumarin 153 is also referred to as Coumarin 540A (Exciton). From [1]. Table I.2. Normalized Emission Intensities for Each Emission Standard (emission wavelength is reported in nm and intensity given in photons/nm) Tryptophan NPO TPB Coumarine 153 DCM LDS 751 nm int. nm int. nm int. nm int. nm int. nm int. 310 0.111 364 0.162 390 0.11 486 0.118 556 0.1 646 0.101 312 0.149 366 0.247 392 0.137 488 0.142 558 0.115 648 0.114 314 0.194 368 0.342 394 0.167 490 0.169 560 0.132 650 0.128 316 0.242 370 0.433 396 0.199 492 0.2 562 0.148 652 0.143 318 0.299 372 0.509 398 0.235 494 0.235 564 0.168 654 0.159 320 0.357 374 0.571 400 0.271 496 0.274 566 0.19 656 0.177 322 0.417 376 0.618 402 0.31 498 0.315 568 0.214 658 0.196 324 0.485 378 0.656 404 0.35 500 0.36 570 0.238 660 0.216 326 0.547 380 0.69 406 0.393 502 0.407 572 0.266 662 0.238 328 0.611 382 0.725 408 0.436 504 0.458 574 0.303 664 0.26 330 0.675 384 0.764 410 0.478 506 0.509 576 0.331 666 0.284 332 0.727 386 0.813 412 0.525 508 0.56 578 0.353 668 0.308 334 0.771 388 0.864 414 0.571 510 0.613 580 0.38 670 0.334 336 0.814 390 0.915 416 0.62 512 0.665 582 0.412 672 0.361 338 0.86 392 0.959 418 0.663 514 0.715 584 0.444 674 0.388 340 0.906 394 0.988 420 0.699 516 0.762 586 0.478 676 0.416 342 0.928 396 0.999 422 0.735 518 0.806 588 0.513 678 0.445 344 0.957 398 1 424 0.773 520 0.847 590 0.548 680 0.474 346 0.972 400 0.991 426 0.805 522 0.882 592 0.584 682 0.504 348 0.996 402 0.978 428 0.834 524 0.914 594 0.62 684 0.534 350 1 404 0.959 430 0.864 526 0.941 596 0.659 686 0.564 352 0.999 406 0.94 432 0.887 528 0.962 598 0.696 688 0.594 354 0.987 408 0.921 434 0.91 530 0.979 600 0.732 690 0.624 356 0.972 410 0.901 436 0.931 532 0.99 602 0.763 692 0.653 358 0.946 412 0.886 438 0.951 534 0.997 604 0.79 694 0.682 360 0.922 414 0.87 440 0.963 536 1 606 0.818 696 0.711 362 0.892 416 0.855 442 0.976 538 1 608 0.842 698 0.738 364 0.866 418 0.835 444 0.985 540 0.996 610 0.869 700 0.765 366 0.837 420 0.803 446 0.991 542 0.991 612 0.893 702 0.791 368 0.798 422 0.773 448 0.998 544 0.981 614 0.915 704 0.815 370 0.768 424 0.743 450 0.999 346 0.97 616 0.934 706 0.839 372 0.728 426 0.708 452 1 548 0.958 618 0.953 708 0.86 374 0.697 428 0.676 454 0.993 550 0.944 620 0.968 710 0.881 376 0.663 430 0.644 456 0.987 552 0.929 622 0.98 712 0.899 378 0.627 432 0.611 458 0.981 554 0.914 624 0.989 714 0.916 380 0.592 434 0.58 460 0.969 556 0.898 626 0.995 716 0.931 382 0.558 436 0.55 462 0.957 558 0.884 628 0.999 718 0.946 384 0.523 438 0.521 464 0.944 560 0.867 630 1 720 0.956 386 0.492 440 0.494 466 0.931 562 0.852 632 0.998 722 0.965 [continued]
PRINCIPLES OF FLUORESCENCE SPECTROSCOPY 875 Table I.2, cont'd 388 0.461 442 0.467 468 0.915 564 0.835 634 0.994 724 0.973 390 0.43 444 0.441 470 0.896 566 0.818 636 0.986 726 0.978 392 0.404 446 0.418 472 0.879 568 0.801 638 0.978 728 0.982 394 0.375 448 0.394 474 0.856 570 0.785 640 0.964 730 0.984 396 0.348 450 0.371 476 0.835 572 0.768 642 0.952 732 0.983 398 0.323 452 0.349 478 0.816 574 0.75 644 0.938 734 0.982 400 0.299 454 0.326 480 0.796 576 0.729 646 0.923 736 0.977 402 0.279 456 0.306 482 0.775 578 0.712 648 0.904 738 0.971 404 0.259 458 0.285 484 0.752 580 0.69 650 0.886 740 0.963 406 0.239 460 0.266 486 0.727 582 0.672 652 0.867 742 0.954 408 0.222 462 0.248 488 0.706 584 0.653 654 0.843 744 0.943 410 0.204 464 0.232 490 0.68 586 0.634 656 0.821 746 0.931 412 0.19 466 0.216 492 0.657 588 0.615 658 0.795 748 0.917 414 0.176 468 0.201 494 0.633 590 0.596 660 0.771 750 0.902 416 0.163 470 0.187 496 0.609 592 0.576 662 0.745 752 0.885 418 0.151 472 0.174 498 0.586 594 0.557 664 0.718 754 0.868 420 0.139 474 0.162 500 0.564 596 0.538 666 0.694 756 0.849 422 0.128 476 0.151 502 0.542 598 0.518 668 0.666 758 0.83 424 0.118 478 0.14 504 0.521 600 0.501 670 0.639 760 0.81 426 0.109 480 0.131 506 0.499 602 0.483 672 0.613 762 0.789 428 0.101 482 0.122 508 0.476 604 0.465 674 0.586 764 0.767 484 0.113 510 0.456 606 0.447 676 0.559 766 0.745 486 0.105 512 0.437 608 0.43 678 0.534 768 0.722 514 0.419 610 0.414 680 0.509 770 0.699 516 0.4 612 0.398 682 0.485 772 0.676 518 0.382 614 0.383 684 0.46 774 0.653 520 0.364 616 0.368 686 0.431 776 0.63 522 0.348 618 0.355 688 0.407 778 0.606 524 0.332 620 0.341 690 0.391 780 0.583 526 0.318 622 0.328 692 0.371 782 0.56 528 0.302 624 0.315 694 0.355 784 0.537 530 0.289 626 0.303 696 0.336 786 0.514 532 0.274 628 0.292 698 0.319 788 0.492 534 0.261 630 0.28 700 0.302 790 0.47 536 0.249 632 0.269 702 0.284 792 0.449 538 0.237 634 0.258 704 0.268 794 0.428 540 0.226 636 0.248 706 0.254 796 0.407 542 0.215 638 0.239 708 0.239 798 0.387 544 0.205 640 0.229 710 0.225 800 0.368 546 0.194 642 0.219 712 0.212 802 0.349 548 0.184 644 0.211 714 0.199 804 0.331 550 0.175 646 0.204 716 0.188 806 0.313 552 0.166 648 0.195 718 0.177 808 0.296 554 0.158 650 0.188 720 0.166 810 0.28 556 0.15 652 0.18 722 0.156 812 0.264 558 0.143 654 0.173 724 0.147 814 0.249 560 0-135 656 0.166 726 0.138 816 0.234 562 0.129 658 0.159 728 0.131 818 0.221 564 0.122 660 0.152 730 0.123 820 0.207 566 0.116 662 0.146 732 0.116 822 0.195 568 0.11 664 0.14 734 0.109 824 0.183 570 0.105 666 0.134 736 0.103 826 0.171 668 0.129 828 0.16 670 0.124 830 0.15 672 0.118 832 0.14 674 0.113 834 0.131 676 0.108 836 0.122 678 0.104 838 0.114 840 0.106 842 0.099 844 0.092
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Principles of Fluorescence Spectros
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Joseph R. Lakowicz Center for Fluor
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Preface The first edition of Princi
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x GLOSSARY OF ACRONYMS DNS dansyl o
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xii GLOSSARY OF ACRONYMS TRES time-
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xiv GLOSSARY OF MATHEMATICAL TERMS
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xvi CONTENTS 2.9.4. Conversion betw
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xviii CONTENTS 6. Solvent and Envir
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xx CONTENTS 10.4.4. Alignment of Po
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xxii CONTENTS 14.7.1. Simulations o
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xxiv CONTENTS 19.12. New Approaches
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xxvi CONTENTS 25.3. Optical Propert
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2 INTRODUCTION TO FLUORESCENCE Figu
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6 INTRODUCTION TO FLUORESCENCE inst
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10 INTRODUCTION TO FLUORESCENCE Fig
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12 INTRODUCTION TO FLUORESCENCE ns,
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14 INTRODUCTION TO FLUORESCENCE 1.7
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24 INTRODUCTION TO FLUORESCENCE due
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28 INSTRUMENTATION FOR FLUORESCENCE
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3 Fluorescence probes represent the
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PRINCIPLES OF FLUORESCENCE SPECTROS
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98 TIME-DOMAIN LIFETIME MEASUREMENT
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100 TIME-DOMAIN LIFETIME MEASUREMEN
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5 In the preceding chapter we descr
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6 Solvent polarity and the local en
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238 DYNAMICS OF SOLVENT AND SPECTRA
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278 QUENCHING OF FLUORESCENCE 8.1.
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280 QUENCHING OF FLUORESCENCE Figur
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282 QUENCHING OF FLUORESCENCE ly ev
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290 QUENCHING OF FLUORESCENCE relat
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298 QUENCHING OF FLUORESCENCE σ ar
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320 QUENCHING OF FLUORESCENCE 47. R
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322 QUENCHING OF FLUORESCENCE 113.
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328 QUENCHING OF FLUORESCENCE P8.3.
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330 QUENCHING OF FLUORESCENCE P8.11
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332 MECHANISMS AND DYNAMICS OF FLUO
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10 Measurements of fluorescence ani
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444 ENERGY TRANSFER Figure 13.1. Fl
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446 ENERGY TRANSFER wavelength is e
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448 ENERGY TRANSFER Table 13.1. Cal
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450 ENERGY TRANSFER Figure 13.6. Ab
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452 ENERGY TRANSFER safe for many p
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454 ENERGY TRANSFER Figure 13.12. P
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456 ENERGY TRANSFER Figure 13.16. E
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458 ENERGY TRANSFER Figure 13.21. R
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460 ENERGY TRANSFER Figure 13.24. R
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462 ENERGY TRANSFER tiple acceptors
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464 ENERGY TRANSFER Figure 13.29. A
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466 ENERGY TRANSFER Figure 13.33. F
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468 ENERGY TRANSFER Table 13.3. Rep
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470 ENERGY TRANSFER 55. Clegg RM. 1
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472 ENERGY TRANSFER Tong AK, Jockus
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474 ENERGY TRANSFER Figure 13.39. O
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14 In the previous chapter we descr
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16 The biochemical applications of
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578 TIME-RESOLVED PROTEIN FLUORESCE
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608 MULTIPHOTON EXCITATION AND MICR
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19 Fluorescence sensing of chemical
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676 NOVEL FLUOROPHORES Figure 20.1.
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678 NOVEL FLUOROPHORES Figure 20.7.
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680 NOVEL FLUOROPHORES Figure 20.12
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698 NOVEL FLUOROPHORES 33. Acherman
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700 NOVEL FLUOROPHORES 103. Demas J
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702 NOVEL FLUOROPHORES 176. Montalt
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21 During the past 20 years there h
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22 Fluorescence microscopy is one o
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758 SINGLE-MOLECULE DETECTION Figur
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766 SINGLE-MOLECULE DETECTION small
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770 SINGLE-MOLECULE DETECTION Table
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786 SINGLE-MOLECULE DETECTION face
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788 SINGLE-MOLECULE DETECTION TCSPC
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790 SINGLE-MOLECULE DETECTION 53. H
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792 SINGLE-MOLECULE DETECTION Ke PC
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794 SINGLE-MOLECULE DETECTION Polar
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24 In the previous chapter we descr
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Page 831 and 832: PRINCIPLES OF FLUORESCENCE SPECTROS
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Page 881: Appendix I Corrected Emission Spect
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