INFINITY ANALYZE User's Manual - Spectra Services

Lumenera INFINITY ANALYZE
User’s Manual Release 6.0.0
If the Ctrl key is down when the right mouse button is clicked, the nucleus will be
separated into two (or occasionally more) nucleuses.
Double click the right mouse button in an area of the image that has not been
highlighted, to complete the segmentation and complete the counting process.
During the process, spaces within the nucleus with radius less than the closing
parameter specified (in pixels) will be filled, and boundaries with jumps less than the
closing parameter specified (in pixels) will be trimmed. When the counting process
finishes, the nucleus will no longer be highlighted with the overlay color, but the nucleus
boundary and index number will be shown. All areas matching the specified tolerance
criteria will be identified by a boundary and counted. The total number of cells will be
added to the Measurement Result, along with the total area occupied by the identified
regions. (Note: The boundary graphic is smoothed for improved appearance)
The above process may be repeated on the same image, for improved control over the
results. For example, during the first attempt the left mouse button is used for
segmentation. While on a second call to the Cell Counting function on the same image
could involve using the right mouse button to remove outlying pixels. When the same
image is counted again, previous result will be replaced by the new one.
Image segmentation results from the cell count operation are also reported in the
Photometry auxiliary window. The pixel intensity values are presented along with pixel
counts for each of the Red, Green, and Blue channels based on their actual values,
along with summary statistics. Enable the Photometry auxiliary window and select the
Segment tab to display the results of the cell count operation. This data can be copied
into a spreadsheet using a drag & drop operation.
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