Manual of basic techniques for a health laboratory - libdoc.who.int

Contentsix10.1.3 Method 32210.1.4 Results 32410.2 Estimation of urea concentration in blood: diacetyl monoxime/thiosemicarbazide method 32510.2.1 Principle 32510.2.2 Materials and reagents 32510.2.3 Method 32610.2.4 Results 32711. Immunological and serological techniques 32811.1 Introduction to immunology 32811.1.1 Antibodies 32811.1.2 Antigens 32911.1.3 Antigen–antibody interactions 33011.2 Principle of immunochemical techniques 33011.2.1 Primary binding tests 33011.2.2 Secondary binding tests 33211.3 Determination of rheumatoid factors by the latex-agglutinationtechnique 33611.3.1 Materials and reagents 33611.3.2 Method 33611.4 Tests for the determination of anti-streptolysin O antibodies 33611.4.1 Anti-streptolysin O test (ASOT) 33611.4.2 Latex agglutination 33811.5 Determination of b-human chorionic gonadotropin (b-hCG) in urineby the agglutination inhibition technique 33911.5.1 Materials and reagents 33911.5.2 Method 33911.6 Quantitative determination of IgA, IgG and IgM by radialimmunodiffusion 33911.6.1 Materials and reagents 33911.6.2 Method 34011.7 Tests for the determination of HIV antibodies 34111.7.1 ELISA 34111.7.2 Dipstick test 34211.8 Tests for hepatitis virus infection 34211.8.1 ELISA for hepatitis B surface antigen 34311.8.2 Dipstick test for hepatitis B surface antigen 34411.9 Dipstick test for falciparum malaria 34411.9.1 Materials and reagents 34411.9.2 Method 34511.10 Tests for syphilis infection 34611.10.1 RPR test 34711.10.2 TPHA test 348Annex: Reagents and their preparation 350Index 369