Manual of basic techniques for a health laboratory - libdoc.who.int

IndexNote: Page numbers in bold refer to main entries; italicized page numbers refer toillustrations.Acidscorrosive injuries from 98–99handling precautions 97Acquired immunodeficiency syndrome(AIDS) (see also Humanimmunodeficiency virus) 313,320“Actinomycetes”, identification 200,201, 204African trypanosomiasis 182–192,184–187, 259–260, 260, 263Agglutination techniques 333–334,333–334, 336, 336, 339CATT 188–192, 189–192AHD, see Alkaline haematin DAIDS, see Acquired immunodeficiencysyndromeAlbert stain 201, 201–202, 351Albumin, urine 237Alcohols, disinfection 85Alembics, distilled water 25, 25Alkaline haematin D (AHD) 276–279,352Alkaliscorrosive injuries from 99–100handling precautions 97Allen & Ridley sedimentationtechnique 153–154, 154Amies transport medium 352–353Amoebae 105cysts 118–120, 119–121, 123motile forms 111–114, 113–115pathogenicity 111Anaemias 284, 287–288, 292, 293, 313abnormal erythrocytes 305–310,306–310sickle-cell 306, 307, 307, 308–310,314–316, 315Analytical balances 32, 68–69, 69Ancylostoma duodenale (hookworm) 106adults 148, 150eggs 126, 127, 128, 133, 152larvae 157, 158Anisocytosis 309, 309Anthrax (Bacillus anthracis) 204, 204,219Antibodies 328–329, 329antigen interactions 330immunochemical techniques 330–335tests 331–336, 336–349, 344–346,348–349Anticoagulants 42–43, 266, 357Antigens 329–330antibody interactions 330immunochemical techniques 330–335tests 331–336, 336–349, 344–346,348–349Anti-streptolysin O test (ASOT)336–338Applicators, wooden 36Ascaris lumbricoides (roundworm) 106adults 146, 147eggs 126, 127–128, 133, 133, 152ASOT, see Anti-streptolysin O testAspirationbody cavity fluids 218–220buboes 218, 219lymph nodes 183–185, 184–185Assays, immunology 330–331, 330–331,343–344Autoclaves 33, 86–87, 86–88Bacillus anthracis 204, 204, 219Bacteria 197–224anthrax 220body cavity fluids 218–220diarrhoeal diseases 105leprosy 220–224, 221–223smears 199–201sputum/throat specimens 204–207,205–207staining techniques 199–201,199–204, 204stool specimens 216–218, 217urine specimens 240, 249–254,250–253urogenital specimens 209–211,210–211, 215–216Bacteriological index (BI) 223–224Bacteriological tests (see also Bacteria)equipment 37–38laboratory registers 47, 50Balances, laboratory 32, 66–69, 67–69Balantidium coli 105, 116, 117, 121, 121Base units, SI 3Basophilic staining 305Basophilic stippling, erythrocytes 309,310Basophils, polymorphonuclear 310,311, 311Batteries, electrical supply 14–15Battery-operated centrifuges 71Benedict solution 236, 236–237, 353Benzoic acid 358369