Manual of basic techniques for a health laboratory - libdoc.who.int

360 Manual of basic techniques for a health laboratoryLoeffler methylene blue (No. 35)Methylene blue 0.5gAbsolute ethanol (CH 3CH 2OH)30mlPotassium hydroxide (KOH), 200g/l (20%) solution(No. 45)0.1mlDistilled waterq.s. 100mlDissolve the methylene blue in 30ml of distilled water and transfer the solution toa clean brown bottle. Add the potassium hydroxide, ethanol and the remainder ofthe distilled water and mix well. Label the bottle “LOEFFLER METHYLENEBLUE” and write the date. Store in a dark place at room temperatures (20–25°C).Lugol iodine, 1g/l (0.1%) solution (No. 36)Iodine1 gPotassium iodide (KI)2 gDistilled water300mlGrind the dry iodine and potassium iodide in a mortar. Add distilled water, a fewmillilitres at a time, and grind thoroughly after each addition until the iodine andiodide dissolve. Rinse the solution into an amber glass bottle with the remainder ofthe distilled water.Alternatively, measure 300ml of distilled water in a cylinder. First dissolve thepotassium iodide in about 30ml of the distilled water. Add the iodine and mix untildissolved. Add the remainder of the distilled water and mix well. Store in a brownbottle.Label the bottle “LUGOL IODINE 0.1% SOLUTION” and write the date.Lugol iodine, 5g/l (0.5%) solution (No. 37)Iodine5 gPotassium iodide (KI)10gDistilled waterq.s. 300mlGrind the dry iodine and potassium iodide in a mortar. Add distilled water, a fewmillilitres at a time, and grind thoroughly after each addition until the iodine andiodide dissolve. Rinse the solution into an amber glass bottle with the remainder ofthe distilled water.Alternatively, measure 300ml of distilled water in a cylinder. First dissolve thepotassium iodide in about 30ml of the distilled water. Add the iodine and mix untildissolved. Add the remainder of the distilled water and mix well. Store in a brownbottle.Label the bottle “LUGOL IODINE 0.5% SOLUTION” and write the date.May–Grünwald stain (No. 38)May–Grünwald powder5 gMethanolq.s. 1000mlRinse out a clean 1000-ml bottle with methanol. Add a few clean dry glass beads.Add the staining powder and methanol. Mix well to dissolve the stain. Label thebottle “MAY-GRÜNWALD STAIN” and write the date.The stain is improved by keeping for 1–2 weeks, mixing at intervals. It is importantto prevent moisture from entering the stain during its preparation and storage.