Manual of basic techniques for a health laboratory - libdoc.who.int

370 IndexBI, see Bacteriological indexBilirubin crystals, urine 248, 248Binding tests, immunology 330–335,330–335Biopsy specimens 93, 95–96, 160–163,161–163Blank reagent 353Blastocystis hominis (yeast) 124–125, 127Bleaches, household 84–85Bleeding time, measurement 295–296,295–296Bloodcells 265–266, 265–266clotting 266, 266, 297–298, 297–298CSF specimens 256–259, 256–259disinfection 84–85stool specimens 157–159, 158urine specimens 234, 241–242,241–243, 244Blood flukes (Schistosoma spp.) 106,150–151, 151Blood specimenschemistry registers 47, 49collection 102, 267–270, 267–270,280–282, 281, 286dispatch 92equipment 42–43, 43glucose concentrations 322–325,323–324parasites 159–160, 166–196microfilariae 159, 163–172, 166,167, 169, 171, 173Plasmodium spp. 172–182, 178protozoa 172–194, 178, 185, 187,193–194Trypanosoma spp. 159, 182–194,185–187test-tube cleaning 82thick blood films 173–182, 175, 187,187, 193–194, 193–194thin blood films 175, 299–314,300–304, 306–314urea concentrations 7, 325–327Boric acid 353Bottles 36–37Brilliant cresyl blue 316–317, 318–319,353Broken glass injuries 101Brugia spp. 159–160, 165, 166, 171,173Bubonic plague (Yersinia pestis)203–204, 218, 219Buffered glycerol saline 217–218, 353–354Buffered water 29–31, 31, 354, 361–362Bunsen burners 35Burettes 77, 77Burns, laboratory accidents 100–101Butane gas burners 97Cables, electrical 19Cabot ring bodies 309, 310Calcium crystals, urine 245, 245, 246,246, 247Calcium hydroxide 85Calcium hypochlorite 84–85Calculations (see also Measurement)blood glucose concentration 7,324–325blood urea concentration 327erythrocyte number concentration 6,287–288erythrocyte sedimentation rate292–295, 293–294erythrocyte volume fraction 6,279–287, 280–283, 286–287haemoglobin concentration 7,271–279, 272–273leukocyte number concentration 6,258–259, 258–259, 288–290,288–292leukocyte type number fraction 6,319–321, 320malaria parasites 180–182reticulocyte number concentration/fraction 6, 317–318thrombocyte number concentration7, 321urine protein 238–239Calibrated dropping pipettes 75, 75Calibrationcolorimeters 277, 278, 279, 324spectrophotometers 272–274,272–273, 276, 279Campylobacter spp. 216Candida albicansCSF 257, 261, 261identification 200, 200vaginal discharge 215Capillary bloodbleeding time 295–296, 295–296collection 164, 164, 166, 280–281,281glucose concentration 325Carbol fuchsin solution 354Card agglutination trypanosomiasis test(CATT) 188–192, 189–192Cary–Blair transport medium 216–217,354Casts, urine 240, 243–244, 243–244Catheters 233CATT, see Card agglutinationtrypanosomiasis testCavity body fluids, specimens 218–219Cellophane faecal thick smeartechnique 141–143, 142–143Cellsblood 125, 125, 265–266, 265–266plasma 312, 312polymorphonuclear 310–311, 311,313, 313target 306, 307urine specimens 241–243, 241–243Centrifugation 69, 166–168