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Manual of basic techniques for a health laboratory - libdoc.who.int

Manual of basic techniques for a health laboratory - libdoc.who.int

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116 <strong>Manual</strong> <strong>of</strong> <strong>basic</strong> <strong>techniques</strong> <strong>for</strong> a <strong>health</strong> <strong>laboratory</strong>Fig. 4.21 Trichomonas hominis trophozoitesFig. 4.22 Chilomastix mesnili trophozoitesCytoplasm: greyish-green with:— towards the tapered end: a distinct spiral marking, around which the flagellateturns (figure-<strong>of</strong>-eight)— near the rounded end: a mouth-like cleft (fa<strong>int</strong>ly visible cytostome).Nucleus: one nucleus, easily visible in unstained preparations.Identification <strong>of</strong> motile <strong>for</strong>ms <strong>of</strong> ciliatesBalantidium coli (rare) (Fig. 4.23)Size: about 50mm.Shape: oval, with one pole more rounded than the other.Cilia: covered with many small cilia, which move with rapid strokes.Motility: moves very rapidly in stools, crossing the field in a definite direction andsometimes turning in circles.Cytoplasm: transparent.Nuclei: a large kidney-shaped nucleus next to a small round nucleus.“Mouth”: the cytostome; a sort <strong>of</strong> mouth that contracts and expands, drawing incell debris.Important: If stools are left exposed to the air, without a lid, organisms <strong>of</strong> the infusoriatype may fall on to them from the atmosphere. These may be confused withBalantidium coli.

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