Manual of basic techniques for a health laboratory - libdoc.who.int

136 Manual of basic techniques for a health laboratory3. Gently pull the tape away from the slide and loop it over the end of the spoonhandle, as shown in Fig. 4.57.4. Hold the completed tape swab in your right hand, pressing the slide firmly againstthe spoon.5. Separate the patient’s buttocks with your left hand. Press the end of the spooncovered with tape against the skin round the anus in several places (Fig. 4.58).6. Take the slide and fold the tape back on to it, sticky side down (Fig. 4.59).7. Make sure that the tape is firmly stuck flat to the slide by pressing it with a pieceof cotton wool (Fig. 4.60).8. Examine under the microscope with the condenser aperture reduced, using the¥ 10 objective. Look for eggs of E. vermicularis (see Fig. 4.54).Fig. 4.57 Looping the tape over the end of the spoon handleFig. 4.58 Technique for collecting pinworm eggs from an infant