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Manual of basic techniques for a health laboratory - libdoc.who.int

Manual of basic techniques for a health laboratory - libdoc.who.int

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364 <strong>Manual</strong> <strong>of</strong> <strong>basic</strong> <strong>techniques</strong> <strong>for</strong> a <strong>health</strong> <strong>laboratory</strong>Add the sodium chloride solution to a glass bottle. Add the saponin, mix, and heatuntil it has completely dissolved.Label the bottle “1% SAPONIN IN SALINE” and write the date.Silver nitrate, 17g/l (1.7%) solution (No. 49)Silver nitrate (AgNO 3) 5.1gDistilled waterq.s. 300mlMix until all the silver nitrate has dissolved. Label the bottle “SILVER NITRATE1.7% SOLUTION” and write the date.Warning: Silver nitrate is caustic.Sodium bicarbonate, 20g/l (2%) solution (No. 50)Sodium bicarbonate (NaHCO 3)2gDistilled waterq.s. 100mlLabel the volumetric flask “SODIUM BICARBONATE 2% SOLUTION” andwrite the date.Sodium carbonate, 2gl/l (0.2%) solution (No. 51)Sodium carbonate (Na 2CO 3), anhydrous2 g(or an equivalent quantity <strong>of</strong> one <strong>of</strong> the hydrates)Distilled waterq.s. 100mlLabel the volumetric flask “SODIUM CARBONATE 0.2% SOLUTION” andwrite the date.Sodium carbonate, 50g/l (5%) solution (No. 52)Sodium carbonate (Na 2CO 3), anhydrous5 g(or an equivalent quantity <strong>of</strong> one <strong>of</strong> the hydrates)Distilled waterq.s. 100mlLabel the volumetric flask “SODIUM CARBONATE 5% SOLUTION” and writethe date.Sodium chloride, 8.5g/l (0.85%) solution (isotonic saline)(No. 53)Sodium chloride (NaCl) 8.5gDistilled waterq.s. 1000mlLabel the volumetric flask “SODIUM CHLORIDE 0.85% SOLUTION” and writethe date.Sodium citrateSee Trisodium citrate.Sodium hydrogen carbonateSee Sodium bicarbonate.Sodium hydroxide, 0.01mol/l solution (No. 54)Sodium hydroxide (NaOH) pellets3 gDistilled waterq.s. 100ml

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