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Manual of basic techniques for a health laboratory - libdoc.who.int

Manual of basic techniques for a health laboratory - libdoc.who.int

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302 <strong>Manual</strong> <strong>of</strong> <strong>basic</strong> <strong>techniques</strong> <strong>for</strong> a <strong>health</strong> <strong>laboratory</strong>Fig. 9.67 Draw the spreader back until it touches the drop <strong>of</strong> bloodFig. 9.68 Allow the blood to run along the edge <strong>of</strong> the spreaderFig. 9.69 Push the spreader to the end <strong>of</strong> the slide witha smooth movementFig. 9.70 Correctly (a) and incorrectlyprepared (b) blood films6. Check that the film is satisfactory as shown in Fig. 9.70 (a).● There should be no lines extending across or down through the film.● The film must be smooth at the end, not ragged and lined as shown inFig. 9.70 (b).● The film must not be too long.● The film must not be too thick.● The film must not contain holes because a greasy slide has been used.A well-spread film is <strong>of</strong> great importance. A badly spread film will give the wrongleukocyte type number fractions and make it impossible to report erythrocytemorphology.

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