376 IndexLabelling (continued)specific reagents 350–368specimens <strong>for</strong> transport 94Laboratory workers, responsibilities 2Lactophenol cotton blue mountingsolution 359Lamps, microscopes 58–60, 59Lancet flukes (Dicrocoelium spp.) 106,128, 134, 134Lancets 36Larvae (see also Micr<strong>of</strong>ilariae) 156–157,158stool specimens 109urine specimens 248Latex agglutination <strong>techniques</strong> 336,336, 338Lead batteries 14–15Lead poisoning 255, 309Leishman stain 299, 303, 305, 354, 359Leishmaniasis (Leishmania spp.)194–196, 196Lenses, microscopes 53–54, 54Leprosy (Mycobacterium leprae)220–224, 221–223Leukaemia 285, 291, 313Leukocytes 125, 125, 265, 265, 310–314,311–314CSF specimens 255–257, 255–257malaria 180–182number concentration 6, 265, 288–290, 288–292type number fraction 6, 259, 319–320,320urine specimens 240, 242, 242–243Leukocytosis 291Leukopenia 291Liquidsheating precautions 97measurement/dispensing 73–77,73–77Litre, measurement 4–5Liver diseases 306, 307Liver flukes, see FlukesLoa spp. 159–160, 163, 165, 172Loeffler methylene blue 204, 360Lugol iodine 360Lutzomyia longipalpis 194Lymphatic system, filarial worms 159Lymph nodes, aspiration 183–185,184–185Lymphoblasts 313, 314Lymphocytes 311–313, 311–313Lymphocytosis 320Lymphopenia 320Lysis time, blood specimens 297–298,297–298Lysol 84–85Macrocytes 307, 308Macroscopic examination, see DirectexaminationMadura foot, see MycetomaMagnification, microscopes 53–56,54–56Ma<strong>int</strong>enance (see also Repairs)batteries 15burettes 77centrifuges 83incubators 83lead batteries 15microscopes 64–66, 65water-baths 83Malaria (see also Plasmodium spp.) 159,172–182, 178, 291, 309blood cells 312, 313, 320dipstick test 344–346, 345–346Male patients, urogenitalspecimens 197, 207–208,209–210Mammomonogamus laryngeus 205Mancini technique, see RadialimmunodiffusionMansonella spp. 159–160, 163, 163, 165,172Markers, hepatitis B virus 342–343Mass, definition 3, 5May–Grünwald stain 299, 304, 305,354, 360Mean erythrocyte haemoglobinconcentration 285–286Measles 312, 313, 320Measurement (see also Calculations;Number concentrations; Numberfractions) 2–7balances 32, 66–69, 67–69bleeding time 295–296, 295–296blood clotting 297–298, 297–298ESR 292–295, 293–294helm<strong>int</strong>h eggs 126–127liquids 73–77, 73–77lysis time 297–298, 297–298metric system 2–7ocular micrometers 63, 63–64pH, urine 235, 235–236protozoa cysts 123quantities 2–3traditional system 5–7Megakaryocytes 266, 314, 314Meker burners 35Meningitis 255, 259common causes 256CSF examination 197, 260–261,260–261, 263tuberculous 257, 259, 261, 263Meningonema spp. 159Mercurothiolate, see ThiomersalMetagonimus yokogawai 106, 128, 139,139Meters, electricity 16, 16Methylene blue solution 361Metric system 2–7Microcytes 307, 307Micr<strong>of</strong>ilariae 159–172, 163, 166,171–172, 249
Index 377Microhaematocrit equipment 37Micrometers, ocular 63, 63–64Micropipettes 38–39, 74, 74–75Microscopes 32, 38, 53–57, 53–66,59–63, 65adjustment system 57, 57–58cleaning 64–65, 65focusing 61, 62–63illumination system 56–57, 56–57, 59,59ma<strong>int</strong>enance 64–66, 65mirrors 56, 56, 60, 60precautions 65–66setting up 58–61, 59–61slides 37, 79–80, 80Microscopic examinationacid-fast bacilli 203Bacillus anthracis 204, 204, 220body cavity fluid 219–220Corynebacterium diphtheriae 202CSF 257–261, 258–261fungi 226, 226, 227, 229, 229gonorrhoea 208, 209–210Gram-stained organisms 200–201,200–201, 253Leishmania spp. 195–196, 196leukocyte type number fraction319–320malaria parasites 178, 178–182micr<strong>of</strong>ilariae in blood 164, 166–167,166–172, 169, 171–172micr<strong>of</strong>ilariae, Onchocerca volvulus162–163, 162–163mycetoma pus specimens 227Mycobacterium leprae 223, 223–224pityriasis versicolor 229, 229protozoa cysts 124, 124–125reticulocyte numbers 317, 317–318semen specimens 212–214, 212–215sickle-cell anaemia 315, 315–316sputum/throat specimens 206stool specimens 107–109, 108–109syphilis treponemes 211, 211thin blood films 305–314, 306–314thrombocyte number concentration321Trypanosoma spp. 185–187, 185–188urine 241–248, 241–248, 250–253,251–254vaginal discharge specimens 215watery stools 216, 217Yersinia pestis 204Moledefinition 3substance concentration 5Molecular weight 3Monocytes 311–312, 312Morphological index, Mycobacteriumleprae 224Mortars/pestles 35Motile <strong>for</strong>ms (see also Trophozoites)protozoa 111–117, 111–118Motility, spermatozoa 214Mott cells 259, 260Mycetoma, pus examination 226–227Mycobacteriumleprae 202, 220–224, 221–223tuberculosis 197, 206Mycology (see also Fungi) 225–229, 226,228–229Myel<strong>of</strong>ibrosis 309Myeloma 237, 312Nail specimens, dispatch 93Nasal specimens, leprosy 220, 222,223–224, 223Necator americanus (hookworm)adults 148, 150eggs 126, 128, 140, 140, 152larvae 157, 158Needlesdisposable 36reusable 81, 81, 87, 87Neisseria meningitidis 260, 260, 264Neonates (see also Children; Infants)erythrocyte number concentration287erythrocyte volume fraction 284immune system 328leukocyte number concentration 291leukocyte type number fraction 320normal haemoglobin 275Nephelometry, immunology 335Nephrotic syndrome 247Neubauer counting chambers 259, 288,288–291, 290Neutral red 361Neutropenia 320Neutrophilia 320Neutrophils, polymorphonuclear310–311, 311, 313, 313New glassware, cleaning 77Nickel–cadmium (Ni–Cd) batteries15Normoblasts, see Nucleated erythrocytesNucleated erythrocytes 292, 310, 310Number concentrations 5–7erythrocytes 265, 284, 287–288leukocytes 265, 285, 288–290,288–292leukocytes in CSF 258–259, 258–259reticulocytes 316–319thrombocytes 266, 321Number fractions 5–6leukocyte type 6, 259, 319, 319–321reticulocyte 6, 316–319, 317Occult blood, stools 157–159, 159Ocular micrometers 63, 63–64Oil droplets, identification 145, 145Onchocerca volvulus 160–163, 161–163,165, 249Oocysts, Cryptosporidium spp. 123–124,124
- Page 1 and 2:
M A N U A LO F B A S I CTECHNIQUESF
- Page 3 and 4:
ContentsiManualofbasic techniques f
- Page 5 and 6:
ContentsiiiContentsPrefacex1. Intro
- Page 8 and 9:
viManual of basic techniques for a
- Page 10 and 11:
viiiManual of basic techniques for
- Page 12 and 13:
xManual of basic techniques for a h
- Page 14 and 15:
2 Manual of basic techniques for a
- Page 16 and 17:
4 Manual of basic techniques for a
- Page 18 and 19:
6 Manual of basic techniques for a
- Page 20 and 21:
8 Manual of basic techniques for a
- Page 22 and 23:
10 Manual of basic techniques for a
- Page 24 and 25:
12 Manual of basic techniques for a
- Page 26 and 27:
14 Manual of basic techniques for a
- Page 28 and 29:
16 Manual of basic techniques for a
- Page 30 and 31:
18 Manual of basic techniques for a
- Page 32 and 33:
20 Manual of basic techniques for a
- Page 34 and 35:
22 Manual of basic techniques for a
- Page 36 and 37:
24 Manual of basic techniques for a
- Page 38 and 39:
26 Manual of basic techniques for a
- Page 40 and 41:
28 Manual of basic techniques for a
- Page 42 and 43:
30 Manual of basic techniques for a
- Page 44 and 45:
32 Manual of basic techniques for a
- Page 46 and 47:
34 Manual of basic techniques for a
- Page 48 and 49:
36 Manual of basic techniques for a
- Page 50 and 51:
38 Manual of basic techniques for a
- Page 52 and 53:
40 Manual of basic techniques for a
- Page 54 and 55:
42 Manual of basic techniques for a
- Page 56 and 57:
44 Manual of basic techniques for a
- Page 58 and 59:
46 Manual of basic techniques for a
- Page 60 and 61:
48 Manual of basic techniques for a
- Page 62 and 63:
50 Manual of basic techniques for a
- Page 64 and 65:
52 Manual of basic techniques for a
- Page 66 and 67:
54 Manual of basic techniques for a
- Page 68 and 69:
56 Manual of basic techniques for a
- Page 70 and 71:
58 Manual of basic techniques for a
- Page 72 and 73:
60 Manual of basic techniques for a
- Page 74 and 75:
62 Manual of basic techniques for a
- Page 76 and 77:
64 Manual of basic techniques for a
- Page 78 and 79:
66 Manual of basic techniques for a
- Page 80 and 81:
68 Manual of basic techniques for a
- Page 82 and 83:
70 Manual of basic techniques for a
- Page 84 and 85:
72 Manual of basic techniques for a
- Page 86 and 87:
74 Manual of basic techniques for a
- Page 88 and 89:
76 Manual of basic techniques for a
- Page 90 and 91:
78 Manual of basic techniques for a
- Page 92 and 93:
80 Manual of basic techniques for a
- Page 94 and 95:
82 Manual of basic techniques for a
- Page 96 and 97:
84 Manual of basic techniques for a
- Page 98 and 99:
86 Manual of basic techniques for a
- Page 100 and 101:
88 Manual of basic techniques for a
- Page 102 and 103:
90 Manual of basic techniques for a
- Page 104 and 105:
Table 3.2 Dispatch of specimens 92
- Page 106 and 107:
94 Manual of basic techniques for a
- Page 108 and 109:
96 Manual of basic techniques for a
- Page 110 and 111:
98 Manual of basic techniques for a
- Page 112 and 113:
100 Manual of basic techniques for
- Page 114 and 115:
102 Manual of basic techniques for
- Page 116 and 117:
104 Manual of basic techniques for
- Page 118 and 119:
106 Manual of basic techniques for
- Page 120 and 121:
108 Manual of basic techniques for
- Page 122 and 123:
110 Manual of basic techniques for
- Page 124 and 125:
112 Manual of basic techniques for
- Page 126 and 127:
114 Manual of basic techniques for
- Page 128 and 129:
116 Manual of basic techniques for
- Page 130 and 131:
118 Manual of basic techniques for
- Page 132 and 133:
120 Manual of basic techniques for
- Page 134 and 135:
122 Manual of basic techniques for
- Page 136 and 137:
124 Manual of basic techniques for
- Page 138 and 139:
126 Manual of basic techniques for
- Page 140 and 141:
128 Manual of basic techniques for
- Page 142 and 143:
130 Manual of basic techniques for
- Page 144 and 145:
132 Manual of basic techniques for
- Page 146 and 147:
134 Manual of basic techniques for
- Page 148 and 149:
136 Manual of basic techniques for
- Page 150 and 151:
138 Manual of basic techniques for
- Page 152 and 153:
140 Manual of basic techniques for
- Page 154 and 155:
142 Manual of basic techniques for
- Page 156 and 157:
144 Manual of basic techniques for
- Page 158 and 159:
146 Manual of basic techniques for
- Page 160 and 161:
148 Manual of basic techniques for
- Page 162 and 163:
150 Manual of basic techniques for
- Page 164 and 165:
152 Manual of basic techniques for
- Page 166 and 167:
154 Manual of basic techniques for
- Page 168 and 169:
156 Manual of basic techniques for
- Page 170 and 171:
158 Manual of basic techniques for
- Page 172 and 173:
160 Manual of basic techniques for
- Page 174 and 175:
162 Manual of basic techniques for
- Page 176 and 177:
164 Manual of basic techniques for
- Page 178 and 179:
166 Manual of basic techniques for
- Page 180 and 181:
168 Manual of basic techniques for
- Page 182 and 183:
170 Manual of basic techniques for
- Page 184 and 185:
172 Manual of basic techniques for
- Page 186 and 187:
174 Manual of basic techniques for
- Page 188 and 189:
176 Manual of basic techniques for
- Page 190 and 191:
178 Manual of basic techniques for
- Page 192 and 193:
180 Manual of basic techniques for
- Page 194 and 195:
182 Manual of basic techniques for
- Page 196 and 197:
184 Manual of basic techniques for
- Page 198 and 199:
186 Manual of basic techniques for
- Page 200 and 201:
188 Manual of basic techniques for
- Page 202 and 203:
190 Manual of basic techniques for
- Page 204 and 205:
192 Manual of basic techniques for
- Page 206 and 207:
194 Manual of basic techniques for
- Page 208 and 209:
196 Manual of basic techniques for
- Page 210 and 211:
198 Manual of basic techniques for
- Page 212 and 213:
200 Manual of basic techniques for
- Page 214 and 215:
202 Manual of basic techniques for
- Page 216 and 217:
204 Manual of basic techniques for
- Page 218 and 219:
206 Manual of basic techniques for
- Page 220 and 221:
208 Manual of basic techniques for
- Page 222 and 223:
210 Manual of basic techniques for
- Page 224 and 225:
212 Manual of basic techniques for
- Page 226 and 227:
214 Manual of basic techniques for
- Page 228 and 229:
216 Manual of basic techniques for
- Page 230 and 231:
218 Manual of basic techniques for
- Page 232 and 233:
220 Manual of basic techniques for
- Page 234 and 235:
222 Manual of basic techniques for
- Page 236 and 237:
224 Manual of basic techniques for
- Page 238 and 239:
226 Manual of basic techniques for
- Page 240 and 241:
228 Manual of basic techniques for
- Page 243 and 244:
7. Examination of urine 231Part III
- Page 245 and 246:
7. Examination of urine 2337. Exami
- Page 247 and 248:
7. Examination of urine 2357.2.3 Me
- Page 249 and 250:
7. Examination of urine 237Table 7.
- Page 251 and 252:
7. Examination of urine 239The anal
- Page 253 and 254:
7. Examination of urine 241MethodCo
- Page 255 and 256:
7. Examination of urine 243Table 7.
- Page 257 and 258:
7. Examination of urine 245Fig. 7.2
- Page 259 and 260:
7. Examination of urine 247Fig. 7.2
- Page 261 and 262:
7. Examination of urine 2497.2.8 De
- Page 263 and 264:
7. Examination of urine 251Reuse of
- Page 265 and 266:
7. Examination of urine 253Fig. 7.4
- Page 267 and 268:
8. Examination of cerebrospinal flu
- Page 269 and 270:
8. Examination of cerebrospinal flu
- Page 271 and 272:
8. Examination of cerebrospinal flu
- Page 273 and 274:
8. Examination of cerebrospinal flu
- Page 275 and 276:
8. Examination of cerebrospinal flu
- Page 277 and 278:
9. Haematology 2659. HaematologyHae
- Page 279 and 280:
9. Haematology 2679.2 Collection of
- Page 281 and 282:
9. Haematology 269Fig. 9.13 Feeling
- Page 283 and 284:
9. Haematology 2719.3 Estimation of
- Page 285 and 286:
9. Haematology 273Table 9.2 Sample
- Page 287 and 288:
9. Haematology 275Fig. 9.25 Checkin
- Page 289 and 290:
9. Haematology 277Calibration of th
- Page 291 and 292:
9. Haematology 279Errors in haemogl
- Page 293 and 294:
9. Haematology 281Fig. 9.28 Micro s
- Page 295 and 296:
9. Haematology 283Fig. 9.34 Centrif
- Page 297 and 298:
9. Haematology 285concentration is
- Page 299 and 300:
9. Haematology 287Measurement techn
- Page 301 and 302:
9. Haematology 289●●●Graduate
- Page 303 and 304:
9. Haematology 2911 litre, so multi
- Page 305 and 306:
9. Haematology 293Fig. 9.45 Materia
- Page 307 and 308:
9. Haematology 295Very high ESR val
- Page 309 and 310:
9. Haematology 2979.9 Observation o
- Page 311 and 312:
9. Haematology 2999.10 Preparation
- Page 313 and 314:
9. Haematology 301Fig. 9.64 Making
- Page 315 and 316:
9. Haematology 303Drying the filmAd
- Page 317 and 318:
9. Haematology 3054. Examine the co
- Page 319 and 320:
9. Haematology 307Fig. 9.77 Normal
- Page 321 and 322:
9. Haematology 309Fig. 9.85 Anisocy
- Page 323 and 324:
9. Haematology 311Fig. 9.92 Polymor
- Page 325 and 326:
9. Haematology 313Immature granuloc
- Page 327 and 328:
9. Haematology 315Fig. 9.104 Mixing
- Page 329 and 330:
9. Haematology 317Fig. 9.108 Prepar
- Page 331 and 332:
9. Haematology 319Heinz bodiesHeinz
- Page 333 and 334:
9. Haematology 3219.14 Determinatio
- Page 335 and 336:
10. Blood chemistry 323Fig. 10.1 De
- Page 337 and 338: 10. Blood chemistry 325Table 10.1 B
- Page 339 and 340: 10. Blood chemistry 32710.2.4 Resul
- Page 341 and 342: 11. Immunological and serological t
- Page 343 and 344: 11. Immunological and serological t
- Page 345 and 346: 11. Immunological and serological t
- Page 347 and 348: 11. Immunological and serological t
- Page 349 and 350: 11. Immunological and serological t
- Page 351 and 352: 11. Immunological and serological t
- Page 353 and 354: 11. Immunological and serological t
- Page 355 and 356: 11. Immunological and serological t
- Page 357 and 358: 11. Immunological and serological t
- Page 359 and 360: 11. Immunological and serological t
- Page 361 and 362: 11. Immunological and serological t
- Page 363 and 364: Annex. Reagents and their preparati
- Page 365 and 366: Annex. Reagents and their preparati
- Page 367 and 368: Annex. Reagents and their preparati
- Page 369 and 370: Annex. Reagents and their preparati
- Page 371 and 372: Annex. Reagents and their preparati
- Page 373 and 374: Annex. Reagents and their preparati
- Page 375 and 376: Annex. Reagents and their preparati
- Page 377 and 378: Annex. Reagents and their preparati
- Page 379 and 380: Annex. Reagents and their preparati
- Page 381 and 382: IndexNote: Page numbers in bold ref
- Page 383 and 384: Index 371Centrifuges 32, 69-72, 69-
- Page 385 and 386: Index 373Endolimax nanus 114, 114,
- Page 387: Index 375Hyaline casts, urine 243,
- Page 391 and 392: Index 379Protozoablood 173-194, 178
- Page 393 and 394: Index 381Smears (continued)preparat
- Page 395 and 396: Index 383Thiomersal-iodine-formalde
- Page 397 and 398: Selected WHO publications of relate