Manual of basic techniques for a health laboratory - libdoc.who.int

Index 379Protozoablood 173–194, 178, 185, 187,193–194intestinal 105–106, 111–117,111–124, 119–122, 124transmission routes 106Public health, laboratory reports 47–48,52Pus casts, urine 243, 244Pus specimensBacillus anthracis 219–220containers 83CSF 256, 256dispatch 92gonorrhoea 210, 210identification 125, 125mycetoma 227–228PVA, see Polyvinyl alcoholPyelonephritis 237Quality assurance 101–102Quality controlAHD reagent 352demineralized water 28–29distilled water 27Quantities, measurement 2–7Quaternary ammonium compounds(QUATS) 85QUATS, see Quaternary ammoniumcompoundsRadial immunodiffusion 335, 335,339–341Radioimmunoassay 330, 330Rapid Field stain, trophozoites 117Rapid plasma reagin (RPR) test,syphilis 346, 347–348, 348Reagents 1, 350–368acetic acid 350–351acetone–ethanol decolorizer 351acid–ethanol for Ziehl–Neelsenstain 351acid reagent 351Albert stain 201, 351alkaline haematin D reagent 276,352Amies transport medium 352–353Benedict solution 353benzoic acid 358blank reagent 353boric acid 353brilliant cresyl blue 353buffered glycerol saline 353–354buffered water, pH 7.2 29–31, 354Carbol fuchsin solution 354Cary–Blair transport medium 354chemical formulae 350colour reagent 367crystal violet, modified Hucker 355Delafield’s haematoxylin stain 355diacetyl monoxime stock solution 367dichromate cleaning solution 355Reagents (continued)disodium hydrogen phosphate stocksolution 361–362Drabkin diluting fluid 355–356EDTA dipotassium salt 356eosin 356expiry dates 46Field stains 117, 356–357fixatives 95, 362–363, 365–366, 368fluoride oxalate anticoagulant 357formaldehyde 357Giemsa stain 357–358glucose reagents 358glycerol–malachite green solution 359hydrochloric acid 359lactophenol cotton blue mountingsolution 359Leishman stain 359Loeffler methylene blue 204, 360Lugol iodine 360May–Grünwald stain 360methylene blue solution 361modified Schaudinn fixative 362neutral red 361Pandy reagent 361phenol red 361phosphate-buffered water 361–362polyvinyl alcohol (PVA) fixative362–363potassium hydroxide 363potassium permanganate 363safranine solution 363saponin 363–364silver nitrate 364sodium bicarbonate 364sodium carbonate 364sodium chloride 364sodium hydroxide 364–365sodium metabisulfite 365streptolysin O 336–338Stuart transport medium, modified365sulfosalicylic acid 365thiomersal–iodine–formaldehyde(TIF) 365–366o-toluidine 322–325, 323–324, 358trisodium citrate 366Türk solution 366urea reagents 325, 366–367Wayson stain 203, 367Willis solution 152, 368Wintrobe solution 368Zenker fixative 368Ziehl–Neelsen stain 202–203, 219,221, 351, 354Records, laboratory 38, 47–52, 223–224Red blood cells, see ErythrocytesReference laboratories, specimendispatch 91–96, 93–94Reference rangesblood glucose concentrations324–325