382 IndexStrongyloides stercoralis (continued)larvae 156–157, 158Stuart transport medium 207, 209, 264,264, 365Subarachnoid haemorrhage 256–257,257, 262, 263Sulfosalicylic acid 365Sulfuric acid, handling 97Supplieselectricity 12–20<strong>laboratory</strong> 33, 34–35, 36–39ordering procedures 46stocktaking 45–46Supplies (continued)storage 45water 24–31Switches, electrical 19, 19SymptomsAfrican trypanosomiasis 183Chagas disease 192–193leishmaniasis 194malaria 172Syphilis (Treponema pallidum) 197,209–211, 211tests 346–349, 348–349Syringes 36, 81, 81, 87, 87Système <strong>int</strong>ernationale, see Units, SITaenia spp. (tapeworm) 106adults 146–148, 147–149eggs 128, 143–144, 144, 152identification 149Tally counters 37Tapeworm, fish (Diphyllobothriumlatum) 106, 128, 134, 134, 152Tapeworm (Taenia spp.)adults 146–148, 147, 149eggs 128, 143–144, 144, 152identification 149Taps, water supply 20–22, 20–22Target cells, erythrocytes 306, 307Techniques (see also Tests)agglutination 188–192, 189–192,333–334, 333–336, 336,338–339anthrax detection 204, 204, 219–220biopsy fixation 95–96blood sample staining 175–176,175–177cellophane faecal thick smear141–143, 142–143CSF examinations 256–264, 256–264fungi detection 225–229, 226,228–229, 261, 261gonorrhoea diagnosis 207–209, 208leishmaniasis diagnosis 196, 195–196leprosy diagnosis 221–223, 221–224micr<strong>of</strong>ilariae detection 164, 164–172,166–167, 169, 171–172oocyst detection 123–124, 124parasite concentration 152–156,152–157Techniques (continued)pinworm eggs examination 135–138,135–137Plasmodium spp. detection 173–182,178Schistosoma mansoni detection141–143, 142–143sedimentation 153–157, 154–156semen examination 212, 212–214smear staining 199–201, 199–204, 204sputum/throat swab examination205–206, 205–206stools examination 107–109, 108–109syphilis diagnosis 210–211, 211,346–349tapeworm detection 147–149,147–148thin blood films 300–304, 300–314,306–314trophozoites staining 117–118Trypanosoma spp. detection 183–194,184–187, 189–194urine protein estimation 238–239Willis solution flotation 152–153,153–154Tests (see also Techniques)African trypanosomiasis 188–192,189–192anthrax 204, 204, 220antibody determination 339–341ASOT 336–338Chagas disease 193–194, 193–194ELISA 341–342, 343–344falciparum malaria 344–346,345–346hepatitis 342–344, 344HIV 341–342, 342immunology 330–335, 330–335latex agglutination 336, 336, 338leishmaniasis <strong>for</strong>mol gel 196Onchocerca volvulus 160–163, 161–163rheumatoid factors determination336, 336sickle-cell anaemia 314–316, 315stools occult blood 157–159, 158syphilis infection 346–349, 348–349Test-tubes 34, 37cleaning 82heating precautions 97holders 35Thalassaemias 306–309Thermometers 35Thick blood filmsChagas disease 193–194, 193–194Plasmodium spp. 173–182, 175Trypanosoma spp. 187, 187Thimerosal, see ThiomersalThin blood films 299–314, 300–304,306–314Plasmodium spp. 173–182, 175preparation 300–303, 300–303staining 303–305, 304
Index 383Thiomersal–iodine–<strong>for</strong>maldehyde(TIF) 109, 110, 365–366Thiosemicarbazide, blood ureaconcentrations 325–327Threadworm, see PinwormThroat specimens 92, 206–209,208–209Thrombocytes 266, 266deficiency 298number concentration 7, 321TIF, see Thiomersal–iodine–<strong>for</strong>maldehydeTimers 35Tinea infection 225–226, 226o-Toluidine reagent 322–325, 323–324,358Tongue depressors 36Toolselectrical repairs 17, 17<strong>laboratory</strong> 38plumbing 20, 20Tourniquets, blood collection 268, 268Toxoplasma gondii 124Traditional system, measurement 5–7Trans<strong>for</strong>mers, electrical 17TransmissionAfrican trypanosomiasis 183blood/skin parasites 160Chagas disease 193<strong>int</strong>estinal parasites 106leishmaniasis 194Transportbiological specimens 91–96, 93–94lead batteries 14specimens, see DispatchTransport media 263–264Amies 352–353buffered glycerol saline 218Cary–Blair 216–217, 354Stuart 207, 209, 264, 264, 365Treponemapallidum 209–210, 346–349pertenue 210–211Trichloroacetic acid 322–323, 353, 358,366Trichomonashominis 115, 116vaginalis 215, 249Trichostrongylus spp. 106, 128, 144,144Trichuris trichiura (whipworm) 106adults 150, 150eggs 126, 128, 144, 144, 152Triple phosphate crystals, urine 245,246Trisodium citrate 366Trophozoites 111–117, 111–118amoebae 113–114, 113–115ciliates 116, 117flagellates 115–116, 115–116staining methods 117–118urine specimens 240, 249Trypanosoma spp. 159, 182–194, 185,187, 193CSF 259–260, 260Trypanosomiasis (see also Trypanosomaspp.) 161, 295African 183–192, 184–187Chagas disease 192–194, 193Tubercle bacilli 253, 253Tuberculosis 197, 295Tuberculous meningitis 257, 259CSF examination 263Ziehl–Neelsen staining 261Tubes (see also Test-tubes)centrifuge 72, 72glass 33, 34, 37, 39–40, 39–42Westergren 37Turbidimetry, immunology 335Türk solution 366Type number fraction, leukocyte 6, 259,319, 319–320Typhoid fever 291, 320Units, SI 2–7Urates, urine 246, 246, 247, 247Urea 7, 325–327Urea reagents 325, 366–367Uric acid crystals, urine 245, 245Urinedisinfection 84neutral 245–246, 248Urine specimens 231–254, 235–236,239–248, 250–253abnormalities detection 241–248,241–248analysis register 47, 49bacteriological examination 251–254,252–253blood 234, 241–242, 241–242casts 243–244, 243–244cells 241–243, 241–243containers 44, 82crystals 236, 245–248, 245–248dispatch 93<strong>for</strong>eign substances 244–245, 245glucose detection 236, 236b-hCG determination 339ketone bodies 239–240, 239–240pH measurement 235, 235–236preservation 234protein detection 236–239Schistosoma haematobium 248, 248,249–251, 250–251Urogenital specimensgonorrhoea 207–208, 208syphilis 209–210, 210vaginal discharge 215–216Vacuum pumps 79, 79Vaginal discharge specimens215–216Vectors (see also Transmission)common filarial parasites 165
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7. Examination of urine 231Part III
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