Manual of basic techniques for a health laboratory - libdoc.who.int

264 Manual of basic techniques for a health laboratoryFig. 8.22 Dispatching CSFspecimens forculture using Stuarttransport medium8.4.2 Method using Stuart transport medium (for the isolation ofNeisseria meningitidis)This is the best method. The medium is supplied in 30-ml bottles that contain 8mlof solid medium (along one side of the flat bottle). The bottles are filled with amixture of air (90%) and carbon dioxide (10%).Follow the instructions given for gonococci in section 5.5.4.If possible, sow centrifuged CSF deposit on the medium (Fig. 8.22); otherwise useuncentrifuged CSF.Preservation time: up to 4 days at room temperature.