374 IndexFormaldehyde–ether sedimentationtechnique 153–154, 154Formol gel test, leishmaniasis 196Formulae, reagents 350FTA-Abs, see Fluorescent treponemalantibody-absorbed testFuchs–Rosenthal countingchamber 258, 258–259Fungi 204, 224–228, 226, 228–229CSF 257, 261, 261identification 124–125, 125, 146, 146urine 240, 248, 248, 252, 253Fuses, electrical 18, 18Gas burners, precautions 97Generators, electrical supply 13Genital specimens, see UrogenitalspecimensGeographical distributionfilarial parasites 160, 165, 172malaria parasites 173Giardia <strong>int</strong>estinalis 115, 115, 121, 121Giemsa stain 175–177, 299–300, 304,305, 357–358Glassware 34–35, 36–37autoclaving 87, 87cleaning 77–81, 78–81disinfection 84heating precautions 97injuries from 101making in the <strong>laboratory</strong> 33, 39–42,39–42stills 25, 26storage 45tubing 37, 39–42, 39–42Globulins, CSF (Pandy test) 262–263,263Glucoseblood concentrations 322–325,323–324CSF specimens 261–262substance concentration 7urine specimens 236, 236Glucose reagents 358Glycerol–malachite green solution 359Gonococcal infection 197, 215urogenital specimens 207–208, 208,253Gonorrhoea, see Gonococcal infectionGraduated conical glasses 77Graduated pipettes 73, 73Gram staining 199–201, 199–201acetone–ethanol decolorizer 351CSF specimens 260–261, 260–261urine specimens 252–253, 253Granular casts, urine 243, 243–244Granulocytes, immature 313, 313Habitat, common filarial parasites 165Haematology (see alsoHaemoglobin) 265–321blood cell types 265–266, 265–266Haematology (continued)blood clotting 297–298, 297–298erythrocytes 279–288, 280–283,286–287ESR 292–295, 293–294leukocytes 319–321, 320microscopes 32registers 47, 49reticulocytes 316–318, 317specimen collection 267–270,267–270test equipment 37, 42–43thin blood films 299–314, 300–304,306–314thrombocytes 321Haemoglobinerythrocyte relationship 284–285estimation 7, 271–279, 272–273, 275,278, 284–285H bodies 319Haemoglobinometers, seeSpectrophotometersHaemophilia 298, 298Haemophilus influenzae 257, 259, 261,261Hairfungal infections 225–226, 227specimen dispatch 93Hand-operated centrifuges 70, 70Hansen disease, see LeprosyHarada–Mori sedimentationtechnique 156–157b-hCG, see b-Human chorionicgonadotropinHeads, centrifuge 70, 71Heinz bodies 319Helm<strong>int</strong>hs<strong>int</strong>estinal 105, 125–152, 129–151,156–159adults 146–152, 147–151eggs 125, 126–144, 129–144larvae 156–159, 158transmission routes 106Heparinized tubes 42Hepatitis, tests 342–344, 344Heterophyes heterophyes 106, 128,138–139, 139HIV, see Human immunodeficiencyvirusHookworm (Ancylostoma duodenale/Necator americanus) 106adults 148, 150eggs 126, 127, 128, 133, 152, 152larvae 157, 158Hot-air ovens 33, 89–90Household bleaches 84–85Howell–Jolly bodies, erythrocytes 309,309b-Human chorionic gonadotropin(b-hCG) 339Human immunodeficiency virus(HIV) 341–342, 342
Index 375Hyaline casts, urine 243, 243Hydatid cysts (Echinococcusgranulosus) 151, 151Hydrochloric acid 359Hygiene, <strong>int</strong>estinal parasites 106Hymenolepis spp. (tapeworm)adults 148, 149eggs 128, 139, 139, 152Hypersegmented polymorphonuclearneutrophils 313, 313Hypochlorite solutions 84–85IdentificationBacillus anthracis 204, 204Balantidium coli 116, 117, 121, 121Blastocystis hominis (yeast) 124–125,125blood parasitesmicr<strong>of</strong>ilariae 163–172, 166, 167,169, 171–172Plasmodium spp. 173–182, 174–176,179–181Trypanosoma spp. 187, 185–194,193–194Corynebacterium diphtheriae 201, 202filariae 159–172, 162–163, 166–167,171–172fungi 124–125, 125, 146helm<strong>int</strong>hsadults 146–152, 147–151eggs 126–144, 129–144larvae 156–158, 158tapeworms 149<strong>int</strong>estinal protozoa 111–117, 111–125,119–122, 124amoebae 113–114, 113–115, 118,120, 120ciliates 116, 121, 121coccidia 122, 122flagellates 115–116, 115–116, 121,121Leishmania spp. 195–196, 196leukocytes 125, 125pollen grains 146, 146pus 125, 125starch granules 144, 144–145Yersinia pestis 204Immun<strong>of</strong>luorescence 332–333, 332Immunoglobulins, see AntibodiesImmunology 328–349, 329–336antibodies 328–329, 329antigens 329–330immune system 328, 329immunochemical <strong>techniques</strong> 330–335tests 336–349Incineration, <strong>laboratory</strong> waste 90, 90–91Incubators, cleaning andma<strong>int</strong>enance 83Infants (see also Children; Neonates)capillary blood collection 280erythrocyte number concentration287Infants (continued)erythrocyte volume fraction 284leukocyte number concentration 291leukocyte type number fraction 320normal haemoglobin 275normal thrombocyte counts 321pinworm egg collection 135–137,135–137reticulocyte number concentration318stool specimens 217urine collection 234Infection sourcesAfrican trypanosomiasis 183blood/skin parasites 160Chagas disease 193<strong>int</strong>estinal parasites 106leishmaniasis 194Infectious mononucleosis 313Injuries, <strong>laboratory</strong> accidents 98–101,99Inoculating loops 197–198, 198Instruments 32disinfection 84storage 45Intestinal parasites 105–159concentration <strong>techniques</strong> 152–156,152–157helm<strong>int</strong>hs 125–152, 129–151adults 146–152, 147–151eggs 125, 126–144, 129–144larvae 156–158, 158protozoa 111–117, 111–124, 119–122,124–125amoebae 113–114, 113–115,118–120, 120ciliates 116, 121, 121coccidia 122, 122cysts 118–124, 119–122, 124–125flagellates 115–116, 115–116, 121,121sedimentation <strong>techniques</strong> 153–157,154–156stool specimens 107–110, 107–111,159–161transmission routes 106Inventories, supplies 46Iodamoeba butschlii 114, 114, 120, 121Iodine 85, 123Iron deficiency 288Isospora belli 124Isotonic saline, see Sodium chlorideKahn tubes 34Kato–Katz technique, Schistosomamansoni infection 141–143,142–143Ketone bodies, urine specimens239–240, 239–240Labellingbiopsy specimens 96
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