Manual of basic techniques for a health laboratory - libdoc.who.int

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38 Manual of basic techniques for a health laboratoryTable 2.2 (cont.)ItemQuantity requiredLoop holders 4Wooden block for loop holders 1Protein standard tubes1 setTest-tube racks, large, for 12 tubes 4Test-tube racks, small, for 12 tubes 4Wooden test-tube holders 2Forceps, stainless steel, for slides 2Bunsen burner for use with butane gas 1Butane gas cylinderas neededTripod with asbestos gauze 1Spatulas, various sizes, for weighing reagents 3Laboratory records and reportsRecord books, hardbacked, large 6Glass-marking pencils, wax, red 12Glass-marking pencils, wax, blue 12Glass marker, diamond point 1Pencils, lead 12Pens, ballpoint, red ink (for recording positive specimens) 2Pens, ballpoint, black or blue ink 3Cellophane tape3 rollsAdhesive tape, white3 rollsLabels for specimen bottles 1000Laboratory request forms (preferably standardized centrally)as neededMiscellaneous equipmentMicroscopes 2Colorimeter 1Water bath 1Refrigerator 1Hot-air oven 1Centrifuge 1Balance 2Deionizer or water still 1Timer, 0–60min, with alarm 1Spirit lamp 1Hammer 1Pliers1 pairPliers, electrician’s1 pairScrewdriver, small 1Screwdriver, medium 1Screwdriver, large 1Round metal file, 5mm 1Small ampoule files 12Saucepan, flat-bottomed with lid, 30cm diameter 1Hot plate 1Pestle (10cm diameter) and mortar 1Bowls, plastic, 50cm ¥ 30cm 3Bucket, plastic, 12 litres 1Rubber safety bulb 4Micropipette, 20ml 1
2. Setting up a peripheral health laboratory 39Table 2.2 (cont.)ItemQuantity requiredMicropipette, 50ml 1Micropipette, 100ml 1Micropipette, 200ml 1Micropipette, 500ml 1Micropipette tips, plastic, disposable, 20mlas neededMicropipette tips, plastic, disposable, 50mlas neededMicropipette tips, plastic, disposable, 100mlas neededMicropipette tips, plastic, disposable, 200mlas neededMicropipette tips, plastic, disposable, 500mlas neededScissors, medium 1Scissors, large 1Vacuum pump, metal 1Thermometer, 0–100 °C 1Stoppers, rubber1 setStoppers, cork1 setCorkscrew 1Test-tube and bottle cleaning brushes (various sizes) 6Filter-paper, 15cm diameter (Whatman’s No. 1 or equivalent)4 boxespH paper, narrow range (6.8–7.2)6 bookspH paper, wide range (0–12)6 booksLens paper2 packetsFine paintbrush, soft camel-hair brush or blower (for cleaning lenses) 1Small rubber bulb (for cleaning lenses) 1Toilet tissue10 rollsTowels and clean ragsas neededImmersion oil6 bottles(10ml each)Fig. 2.41 Marking off the required length of glass tubing using a fileFig. 2.42 Breaking the glass tubing byhandFig. 2.43 Rounding off the ends of theglass tubing by flaming2. Wrap the part to be broken in a cloth. Hold the tube with both hands, onethumb on either side of the etched mark (Fig. 2.42). Snap by pressing with yourthumbs.3. Round off the end of each piece of tubing as shown in Fig. 2.43:— heat the end, holding the tube almost vertical just above the blue flame ofthe burner;
Page 1 and 2: M A N U A LO F B A S I CTECHNIQUESF
Page 3 and 4: ContentsiManualofbasic techniques f
Page 5 and 6: ContentsiiiContentsPrefacex1. Intro
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Table 3.2 Dispatch of specimens 92
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7. Examination of urine 231Part III
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7. Examination of urine 2337. Exami
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7. Examination of urine 2357.2.3 Me
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7. Examination of urine 237Table 7.
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7. Examination of urine 239The anal
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7. Examination of urine 241MethodCo
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7. Examination of urine 243Table 7.
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7. Examination of urine 245Fig. 7.2
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7. Examination of urine 2497.2.8 De
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7. Examination of urine 251Reuse of
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8. Examination of cerebrospinal flu
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9. Haematology 2659. HaematologyHae
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9. Haematology 2679.2 Collection of
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9. Haematology 269Fig. 9.13 Feeling
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9. Haematology 2719.3 Estimation of
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9. Haematology 273Table 9.2 Sample
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9. Haematology 275Fig. 9.25 Checkin
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9. Haematology 277Calibration of th
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9. Haematology 279Errors in haemogl
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9. Haematology 281Fig. 9.28 Micro s
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9. Haematology 283Fig. 9.34 Centrif
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9. Haematology 285concentration is
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9. Haematology 287Measurement techn
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9. Haematology 289●●●Graduate
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9. Haematology 2911 litre, so multi
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9. Haematology 293Fig. 9.45 Materia
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9. Haematology 295Very high ESR val
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9. Haematology 2979.9 Observation o
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9. Haematology 2999.10 Preparation
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9. Haematology 301Fig. 9.64 Making
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9. Haematology 303Drying the filmAd
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9. Haematology 3054. Examine the co
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9. Haematology 307Fig. 9.77 Normal
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9. Haematology 309Fig. 9.85 Anisocy
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9. Haematology 311Fig. 9.92 Polymor
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9. Haematology 313Immature granuloc
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9. Haematology 315Fig. 9.104 Mixing
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9. Haematology 317Fig. 9.108 Prepar
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9. Haematology 319Heinz bodiesHeinz
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9. Haematology 3219.14 Determinatio
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10. Blood chemistry 323Fig. 10.1 De
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10. Blood chemistry 325Table 10.1 B
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10. Blood chemistry 32710.2.4 Resul
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11. Immunological and serological t
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Annex. Reagents and their preparati
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IndexNote: Page numbers in bold ref
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Index 371Centrifuges 32, 69-72, 69-
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Index 373Endolimax nanus 114, 114,
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Index 375Hyaline casts, urine 243,
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Index 377Microhaematocrit equipment
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Index 379Protozoablood 173-194, 178
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Index 381Smears (continued)preparat
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Index 383Thiomersal-iodine-formalde
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