Manual of basic techniques for a health laboratory - libdoc.who.int

11. Immunological and serological techniques 34511.9.2 Method1. Collect a finger-prick sample of blood from the patient.2. Place one drop of blood into a test-tube containing three drops of lysing reagent(Fig. 11.17).3. Place one drop of the lysed blood sample into one of the wells of the test card inthe reaction stand (Fig. 11.18).4. Place the dipstick in the lysed blood until all the blood has been absorbed (Fig.11.19).5. Apply one drop of detection reagent to the base of the dipstick (Fig. 11.20).This reagent consists of a suspension of micelles (phospholipid vesicles) containingsulfo-rhodamine B as a marker coupled to rabbit antibody raised againstHRP-II.6. When the reagent has been absorbed, apply two drops of washing reagent toclear the lysed blood (Fig. 11.21).If the result is positive a thin red line will be left across the dipstick with a brokenline (the reagent control) above it.Fig. 11.17 Adding a blood sample tolysing reagentFig. 11.18 Applying the blood sample to thetest cardFig. 11.19 Placing the dipstick in thelysed bloodFig. 11.20 Applying detection reagent to thedipstick