Manual of basic techniques for a health laboratory - libdoc.who.int

Index 371Centrifuges 32, 69–72, 69–73balancing 72, 72cleaning and maintenance 83tubes 34, 37Cerebrospinal fluid (CSF) 255–264,255–264blood in 256–259, 256–259glucose concentration 7, 261–262laboratory registers 47, 50leukocyte number concentration/fraction 6, 258–259meningitis 260–261, 260–261protein concentration 262, 262specimen containers 44, 82specimen dispatch 92trypanosomes 259–260, 260Chagas disease 192–194, 193–194Chancre, syphilitic 210, 210Chemical formulae, reagents 350Chemicals, storage 45Children (see also Infants; Neonates)Chagas disease 192–194, 193–194erythrocyte number concentration287erythrocyte volume fraction 284Haemophilus influenzae 261, 261leukocyte number concentration291leukocyte type number fraction 320normal haemoglobin 275normal thrombocytes 321reticulocyte number concentration318venous blood collection 267Chilomastix mesnili 115–116, 117, 121,121Chinese liver fluke (Clonorchissinensis) 106, 130, 134, 134Chloramine 85Cholesterol crystals, urine 247, 248Chromatoid bodies 122Ciliatescysts 121, 121motile forms 111, 116, 117pathogenicity 111Cleaning 77–85, 78–82centrifuges 83glassware 77–81, 78–81incubators 83laboratory balances 66–67microscopes 65, 64–65reusable syringes/needles 81specimen containers 81–82, 82Clonorchis sinensis (Chinese liverfluke) 106, 130, 134, 134Clottingblood 266, 266, 297–298, 297–298CSF 257Coccidia 122, 122, 124Colorimeters 32, 271–274AHD method 276–277calibration 277–278, 278, 279, 324Colour reagent 367Communicable diseases, reporting 52Concentration techniques,parasites 152–156, 152–157Concentrationsblood glucose 322–325, 323–324blood urea 325–327CSF glucose 26l–262erythrocyte number 6, 263, 284,287–288haemoglobin 7, 271–279, 284–285leukocyte number 6, 265, 288–290,288–292measurement 4–6reticulocyte number 6, 316–319thrombocyte number 7, 266, 321Condensers, microscopes 56, 56, 58,60–61, 60Confidentiality 2Containerscleaning 81–82, 82“sharps” 96specimens 42–44, 42–44Corrosive injuries, acids/alkalis 98–100Corynebacterium diphtheriae (see alsoDiphtheria)specimen dispatch 207, 207staining techniques 201, 201–202Counters, differential 32Counting chambers 37Fuchs–Rosenthal 258–259, 258–259Neubauer 259, 288, 288–291, 290Counts (see also Numberconcentrations; Numberfractions)parasite eggs 251sperm 214–215Coverslips, slides 80, 80, 81Cresols 83Cresyl blue, see Brilliant cresyl blueCryptococcus neoformans, CSF 257, 261,261Cryptosporidium spp. 123–124, 124,202–203Crystals, urine 236, 240, 245–248,245–248Crystal violet, modified Hucker 355CSF, see Cerebrospinal fluidCulturesdispatch 206–207, 207, 263–264, 264Mycobacterium leprae 223urine 254watery stools 216Cutaneous leishmaniasis 195–196Cuts, laboratory accidents 101Cystine crystals, urine 247, 247Cystshyatid 151, 151protozoa 118–125, 119–122, 124–125stool examination 108, 109yeast 124–125, 125